Comet Assay Image Analysis - WimComet

A web-based quantitative image analysis of comet assays with automated readout of fluorescence intensities

Complete solution for comet assay readout - only a few steps from sample preparation to image analysis
Objective and reproducible analysis
Easy and fast data processing - results within hours
No extra hardware or software needed

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Cat. No.	Description	Pcs./Box
30008-500	WimComet Quantitative Comet Assay Image Analysis, 500 images	500
30008-1000	WimComet Quantitative Comet Assay Image Analysis, 1,000 images	1000
30008-2000	WimComet Quantitative Comet Assay Image Analysis, 2,000 images	2000
30008-5000	WimComet Quantitative Comet Assay Image Analysis, 5,000 images	5000
30008-10000	WimComet Quantitative Comet Assay Image Analysis, 10,000 images	10000
30008-20000	WimComet Quantitative Comet Assay Image Analysis, 20,000 images	20000
30008-50000	WimComet Quantitative Comet Assay Image Analysis, 50,000 images	50000
30008-100000	WimComet Quantitative Comet Assay Image Analysis, 100,000 images	100000

FAQ

FAQ Image Analysis

Applications:

Detection of DNA damage in comet assays (single-cell gel electrophoresis, SCGE)
Studies of genotoxicity
Sperm DNA fragmentation

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WimComet is an image analysis solution that is specially designed to objectively measure the DNA damage in comet assay images, with high precision and reliability. It provides an automated and reproducible quantification of the comet head and tail by using high-end image processing algorithms to detect and compare the signals between the two of them.

Wimasis, the certified partner of ibidi for quantitative image analysis, developed an analysis solution to quantitatively evaluate vessel formation in comet assays. The Image Analysis solution generates fast results, without the need for extra hardware or software.

First, perform your single cell gel electrophoresis and record your fluorescence images. Then, simply upload your data and receive a summary of results, via E-mail, and download the detailed results.

Analysis Data Contains:

Total fluorescence intensity of each single cell
Comet length
Percentage of DNA in the tail
Tail length
Olive tail moment (tail length multiplied by the percentage of DNA in the tail)

Because every experimental setup is unique, the WimComet tool is engineered with the flexibility to adapt to the needs of every researcher. If your image data does not fit the requirements above, send us a quick note, or reach us at: info@ibidi.de, so that we can find a solution that fits your experiment.

Detailed Description of the Analysis:

For the imput data, WimComet uses fluorescence microscopy images of the comet assay, where the bright comets are shown in a dark background. Both images, with one or several comets, can be analyzed.