• Comet Assay Image Analysis - WimComet
  • Comet Assay Image Analysis - WimComet

Comet Assay Image Analysis - WimComet

A web-based quantitative image analysis of comet assays with automated readout of fluorescence intensities

  • Complete solution for comet assay readout - only a few steps from sample preparation to image analysis
  • Objective and reproducible analysis
  • Easy and fast data processing - results within hours
  • No extra hardware or software needed

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Cat. No. Description Pcs./Box
30008-500 WimComet Quantitative Comet Assay Image Analysis, 500 images 500
30008-1000 WimComet Quantitative Comet Assay Image Analysis, 1,000 images 1000
30008-2000 WimComet Quantitative Comet Assay Image Analysis, 2,000 images 2000
30008-5000 WimComet Quantitative Comet Assay Image Analysis, 5,000 images 5000
30008-10000 WimComet Quantitative Comet Assay Image Analysis, 10,000 images 10000
30008-20000 WimComet Quantitative Comet Assay Image Analysis, 20,000 images 20000
30008-50000 WimComet Quantitative Comet Assay Image Analysis, 50,000 images 50000
30008-100000 WimComet Quantitative Comet Assay Image Analysis, 100,000 images 100000

Applications:

  • Detection of DNA damage in comet assays (single-cell gel electrophoresis, SCGE)
  • Studies of genotoxicity
  • Sperm DNA fragmentation
 

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WimComet is an image analysis solution that is specially designed to objectively measure the DNA damage in comet assay images, with high precision and reliability. It provides an automated and reproducible quantification of the comet head and tail by using high-end image processing algorithms to detect and compare the signals between the two of them.

Wimasis, the certified partner of ibidi for quantitative image analysis, developed an analysis solution to quantitatively evaluate vessel formation in comet assays. The Image Analysis solution generates fast results, without the need for extra hardware or software.

First, perform your single cell gel electrophoresis and record your fluorescence images. Then, simply upload your data and receive a summary of results, via E-mail, and download the detailed results.


Analysis Data Contains:

  • Total fluorescence intensity of each single cell
  • Comet length
  • Percentage of DNA in the tail
  • Tail length
  • Olive tail moment (tail length multiplied by the percentage of DNA in the tail)

Because every experimental setup is unique, the WimComet tool is engineered with the flexibility to adapt to the needs of every researcher. If your image data does not fit the requirements above, send us a quick note at info@ibidi.de, so that we can find a solution that fits your experiment.


Detailed Description of the Analysis:

For the imput data, WimComet uses fluorescence microscopy images of the comet assay, where the bright comets are shown in a dark background. Both images, with one or several comets, can be analyzed.


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