• ibidi Pump System
  • ibidi Pump System

ibidi Pump System

A pump system for the cultivation of cells under flow for simulation of blood vessels

  • Ideal simulation of various physiological conditions - continuous unidirectional, oscillating, and pulsatile flow
  • Compatible with the ibidi Heating Systems, all incubators, and incubated microscopes
  • Minimal mechanical stress, minimal amount of medium and supplement needed

ibidi Pump

+



Fluidic Unit

+

PumpControl

+


Optional:
Fluidic Unit Quad




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Cat. No. Description Pcs./Box
10902 ibidi Pump System: ibidi Pump, Fluidic Unit, Perfusion Set, notebook, PumpControl software 1
10903 Fluidic Unit: switching valves for various flow assays, suitable for all Perfusion Sets and channel µ-Slides, stable aluminum housing, exchangeable reservoir holders 1
10904 Fluidic Unit Quad: 4 Fluidic Units on a stable plate, switching valves for various flow assays, suitable for all Perfusion Sets and channel µ-Slides, stable aluminum housing, exchangeable reservoir holders 1
10905 ibidi Pump: accuracy: +/- 1 mbar, pressure range: -100 to +100 mbar, control for up to 4 Fluidic Units, including Pump Control software 1
10908 Notebook: ready to use, pre-configured Windows system, PumpControl software 1
10961 Perfusion Set BLUE, length 15 cm, ID 0.8 mm, 10 ml reservoirs, designed for low flow rates. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes 3
10962 Perfusion Set RED, length 15 cm, ID 1.6 mm, 10 ml reservoirs, designed for high flow rates. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes 3
10963 Perfusion Set WHITE, length 50 cm, ID 0.8 mm, 10 ml reservoirs, designed for low flow rates and microscopy. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes 3
10964 Perfusion Set YELLOW-and-GREEN, length 50 cm, ID 1.6 mm, 10 ml reservoirs, designed for high flow rates and microscopy. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes 3
10965 Perfusion Set YELLOW, length 15 cm, ID 0.5 mm, 2 ml reservoirs, designed for low volumes and µ-Slides with 0.1 mm channel height. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes 3
10966 Perfusion Set BLACK, length 50 cm, ID 0.5 mm, 2 ml reservoirs, designed for low volumes, µ-Slides with 0.1 mm channel height, and microscopy. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes 3
10971 Filter / Reservoir Set, 10 ml, 2 replacement reservoirs with mounted filters, ready to use, sterile 10
10972 Filter / Reservoir Set, 2 ml, 2 replacement reservoirs with mounted filters, ready to use, sterile 10
10974 Filter / Reservoir Set, 50 ml, 2 replacement reservoirs with mounted filters, ready to use, sterile 10
10976 Reservoir Holder for Fluidic Unit, 10 ml 1
10977 Reservoir Holder for Fluidic Unit, 2 ml 1
10978 Reservoir Holder for Fluidic Unit, 50 ml 1
10982 ibidi Pump demo set: 1 Perfusion Set and 5 µ-Slides Luer -Type of your choice 1

Instructions

Instruction Manual ibidi Pump System (PDF)
Instruction Manual PumpControl Software (PDF)
Instruction Manual ibidi Pump System Pulsatile Flow (PDF)
Instruction Manual ibidi Pump System Oscillating Flow (PDF)

Software

Download of Installation CDs for PumpControl Software:

Version 1.5.2

PumpControl Software V. 1.5.2 (zip file, 208 MB)

Version 1.5.1

PumpControl Software V. 1.5.1 for Windows XP/7/8 (zip file, 167 MB)

Version 1.5.0

PumpControl Software V. 1.5.0 for Windows XP (zip file, 162 MB)
PumpControl Software V. 1.5.0 for Windows 7 (zip file, 162 MB)

Version 1.4.4

PumpControl Software V. 1.4.4 for Windows XP (zip file, 94 MB)
PumpControl Software V. 1.4.4 for Windows 7 (zip file, 94 MB)

Certificates

EC Declaration of Conformity (PDF)
TÜV Attestation of Conformity (PDF)

Application Notes

AN 11: Shear Stress and Shear Rates (PDF)
AN 13: HUVECS under Perfusion (PDF)
AN 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF)
AN 25: Serial Connection of Flow Chambers (PDF)
AN 31: Serial Connection of µ-Slide VI 0.4 (PDF)

Movies

MV 10: HUVEC Culture Under Perfusion vs. Static Conditions
MV 17: HUVEC Culture Under Flow
MV 20: The ibidi Pump System
MV 21: Long-Term Cell Culture Under Perfusion

Scientific Poster

Changes in cell-cell contacts of endothelial cells (HUVEC) under long term shear stress conditions (PDF)

Webinar    

Recorded Webinar: Cell Cultivation Under Flow - Introduction
Recorded Webinar: Cell Cultivation Under Flow - New Insights and Applications

Product Flyer

Product Flyer Perfusion Assays (PDF)

FAQ

Cell Culture under Flow

Applications:

 

Components of the ibidi Pump System

ibidi Pump

  • Computer-controlled air pressure pump
  • Operates up to four parallel Fluidic Units per ibidi Pump

PumpControl Software

  • Easy setup of an automated flow control for flow assays
  • Internal calculation for flow rates, shear rates, and shear stress

Fluidic Unit

  • A holder for one Perfusion Set (10 ml) and connected μ-Slide(s)
  • For placement in an incubator
  • Optional: Reservoir Holder for 2 ml and 50 ml Perfusion Sets

Fluidic Unit Quad

  • Combines four Fluidic Units into one system
  • Operated by a single ibidi Pump

Perfusion Set

  • Includes tubing, adapters, and reservoirs to perform flow assays
  • Designed for various flow rates and volumes

Heating Insert Adapter for Perfusion Assay

  • Adapter for the ibidi Heating System
  • For long-term cell studies (several days) under flow conditions

Technical Features:

  • Operates up to four parallel Fluidic Units per ibidi Pump
  • Flow characteristics: all flow types are laminar
  • Some examples:
    • Unidirectional flow simulates veins or small arteries
    • Oscillating flow simulates turbulent conditions at vessel defects
    • Pulsatile flow simulates arterial conditions
  • Flow rate: 0.03 – 35 ml/min
  • Shear stress: 0.3 – 150 dyn/cm2
  • Working volume: 2.5 / 12 ml / 50 ml
  • Suitable for all µ-Slides with Luer adapters
  • Also suitable for home-made flow chambers
  • Compatible with all incubators 
  • Software-controlled  flow rates and shear stress
 

System Overview:

The ibidi Perfusion System Perfectly Reflects the Natural Environment of Cells Under Flow Conditions

The ibidi Pump System consists of two main components: The ibidi Pump (computer controlled air pump) and the Fluidic Unit (holder for cell media reservoirs and μ-Slide, tubing, and electrically controlled valve set). By using this “split” approach, the closed flow setup can be assembled separately and transferred to the microscope or the incubator after cell cultivation, without compromising the sterility of the system. The open architecture using the Luer connectors allows the use of any kind of flow devices. The PumpControl software controls the pressure, and subsequently the shear stress acting on the cells. The system is a fully integrated solution.

By hosting the Fluidic Unit in the incubator, it is still possible to run perfusion assays directly on the microscope. A defined temperature and CO2 concentration is assured, as the fluidic reservoirs are inside the incubator at all times.

 


Principle of Flow Generation

Applying pressured air to the reservoirs of the Fluidic Unit generates the flow in the μ-Slide channels. In order to avoid wasting any medium, the liquid is pumped back and forth from reservoir A to reservoir B by switching the four-fold valve V1.

In state 1, the pressured air is guided to reservoir A while reservoir B is connected to the ambient air pressure. In state 2, it is the other way around. Switching between these states creates a flow of medium between the reservoirs and ensures that they do not run dry. However, to generate a unidirectional flow, a fluidic rectifier is placed between the reservoirs and the μ-Slide. The rectifier is a pinch valve V2 that clamps off two branches of the Perfusion Set while the others are kept open. The synchronous switching of both valves creates a continuous and unidirectional flow without wasting any medium.

 
http://www.ibidi.com/fileadmin/products/instruments/I_1090X_PumpSystem/animated_pump.swf|300|467

Example of a Perfusion Set-up
on a Microscope

Ideal in combination with the ibidi Heating & Incubation System
 

The ibidi Pump System Generates Minimal Mechanical Stress


Application Examples:

rAV CMV-LifeAct-TagRFP-transduced HUVEC, cultivated under 20 dyn / cm² in µ-Slide I 0.4 Luer

 

HUVEC, cultivated over 7 days at 10 dyn / cm². VE-cadherins are stained in green, cell nuclei are stained in blue.

 

Rolling and adhesion of polymorpho-
nuclear leukocytes on LPS-stimu-
lated cerebrovascular endothelial cells in µ-Slide VI 0.4 at 1 dyn / cm².
(Courtesy of G. Cepinskas, Ontario)


Suitable µ-Slides

The following ibidi µ-Slides can be attached to the ibidi Pump System:

Low shear stress

Medium shear stress

High shear stress

NOTE: Custom specific slides can also be used when utilizing the Luer adapters.

Perfusion Sets and µ-Slide Selection

Overview:


Blue
(ID 0.8 mm)
Red
(ID 1.6 mm)
White
(ID 0.8 mm)
Yellow
(ID 1
/Green
.6 mm)
Yellow
(ID 0.5 mm)
Black
(ID .5 mm)
Low shear stress X
X      
High shear stress
X   X X X
Long tubes for
microscopy (50 cm)
    X X   X
Working volume (ml) 12 12 12 12 2.5 2.5
Standard reservoir* (ml) 10 10 10 10 2 2

* By changing the reservoir, different working volumes can be achieved. Reservoirs are available with a syringe volume of 2, 12, and 50 ml.

Details:

    Perfusion Set blue (#10961)
Inner diameter: 0.8 mm Tube length: 15 cm
Total working volume: 11.3 ml Dead volume of tubing: 0.5 ml
  Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.2 Luer 2.5 50 0.83 11.53 4.26 59.14
µ-Slide I 0.4 Luer 2.5 100 1.12 15.30 1.47 20.14
µ-Slide I 0.6 Luer 2.5 150 1.13 15.58 0.68 9.36
µ-Slide I 0.8 Luer 2.5 200 1.15 15.91 0.40 5.53
µ-Slide VI 0.4 0.6 30 1.12 15.25 1.97 26.86
µ-Slide y-shaped 2.8 110 1.08 14.50 2.46 32.98
without slide - (75) 1.18 16.51 - -


    Perfusion Set red (#10962)
Inner diameter: 1.6 mm Tube length: 15 cm
Total working volume: 12.3 ml Dead volume of tubing: 1.5 ml
  Growth
area [cm²]
Channel
volume [µl]
Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.2 Luer 2.5 50 1.28 19.11 6.57 98.02
µ-Slide I 0.4 Luer 2.5 100 2.65 33.17 3.49 43.66
µ-Slide I 0.6 Luer 2.5 150 2.74 34.98 1.65 21.02
µ-Slide I 0.8 Luer 2.5 200 2.75 35.14 0.96 12.21
µ-Slide VI 0.4 0.6 30 2.76 35.33 4.86 62.24
µ-Slide y-shaped 2.8 110 2.59 32.12 5.89 73.05
without slide - (75) 2.79 35.86 - -


  Perfusion Set white (#10963)
Inner diameter: 0.8 mm Tube length: 50 cm
Total working volume: 11.7 ml Dead volume of tubing: 0.9 ml
   Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.2 Luer 2.5 50 0.42 7.15 2.15 36.67
µ-Slide I 0.4 Luer 2.5 100 0.57 8.75 0.75 11.52
µ-Slide I 0.6 Luer 2.5 150 0.61 9.40 0.37 5.65
µ-Slide I 0.8 Luer 2.5 200 0.64 10.11 0.22 3.51
µ-Slide VI 0.4 0.6 30 0.63 9.77 1.11 17.21
µ-Slide y-shaped 2.8 110 0.58 8.94 1.32 20.33
without slide - (75) 0.61 9.43 - -


     Perfusion Set yellow/green (#10964)
Inner diameter: 1.6 mm Tube length: 50 cm
Total working volume: 13.6 ml Dead volume of tubing: 2.8 ml
   Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.2 Luer 2.5 50 1.03 17.00 5.28 87.20
µ-Slide I 0.4 Luer 2.5 100 1.91 25.95 2.51 34.16
µ-Slide I 0.6 Luer 2.5 150 1.98 27.44 1.19 16.49
µ-Slide I 0.8 Luer 2.5 200 2.13 30.25 0.47 10.51
µ-Slide VI 0.4 0.6 30 2.06 28.95 3.63 51.00
µ-Slide y-shaped 2.8 110 1.94 26.60 4.41 60.50
without slide - (75) 2.44 36.02 - -


   Perfusion Set yellow (#10965)*
Inner diameter: 0.5 mm Tube length: 15 cm
Total working volume: 2.5 ml Dead volume of tubing: 0.5 ml
  Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.1 Luer 2.5 25 0.07 1.35 1.42 27.34
µ-Slide VI 0.1 0.17 1.7 0.08 1.44 8.54 153.69

* Perfusion Set only works with PumpControl version 1.5.0 or higher and
   ibidi Pump with hardware version 1.02 or higher and firmware version 1.10 or higher.



   Perfusion Set black (#10966)*
Inner diameter: 0.5 mm Tube length: 50 cm
Total working volume: 2.7 ml Dead volume of tubing: 0.7 ml
  Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.1 Luer 2.5 25 0.03 0.61 0.61 12.36
µ-Slide VI 0.1 0.17 1.7 0.03 0.62 3.20 66.17

* Perfusion Set only works with PumpControl version 1.5.0 or higher and
   ibidi Pump with hardware version 1.02 or higher and firmware version 1.10 or higher.


Shane R. McSweeney, Cardiovascular Division, BHF Centre of Research Excellence, School of Medicine, King’s College London, United Kingdom

"The µ-Slide I Luer facilitates the culture of endothelial cells under defined complex flow patterns. We are particularly interested in the effects of oscillatory shear stress on redox signaling pathways, and the ibidi Air Pump and Fluidic Units are ideal systems for this purpose. The µ-Slides are convenient for both live and fixed cell imaging as well as the collection of protein and RNA samples and other cell analyses. The system has allowed us to easily investigate physiological shear patterns in vitro without a background in engineering.”

Shane R. McSweeney
Cardiovascular Division
BHF Centre of Research Excellence
School of Medicine
King’s College London
United Kingdom

http://www.kcl.ac.uk/medicine/research/divisions/cardio/bhf/index.aspx


Dr. Nynke van den Akker, Maastricht University, Maastricht, The Netherlands

“We've been working with the ibidi Pump System and Labware for over 5 years now and have recommended it to numerous colleagues as well. The plastics are ideal for cell culture and all forms of imaging, including automated imaging (easy to focus) and all sorts of fluorescence (clear, optically superb plastics). The ibidi Pump System in fact made the endothelial cell under flow the default of our lab! And above all, the technical support is always fast and efficient.”

Dr. Nynke van den Akker
Department of Cardiology
Maastricht
University
Maastricht
The Netherlands
www.maastrichtuniversity.nl/web/show/id=74913/langid=42

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