ibidi Pump System

A pump system for the cultivation of cells under flow for simulation of blood vessels

Ideal simulation of various physiological conditions - continuous unidirectional, oscillating, and pulsatile flow
Fully integrated solution, also for microscopy - compatible with the ibidi Heating Systems, all incubators, and incubated microscopes
Complete setup under sterile circular conditions - minimal mechanical stress, minimal amount of medium and supplement

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Cat. No.	Description	Pcs./Box
10902	ibidi Pump System: ibidi Pump, Fluidic Unit, Perfusion Set, notebook, PumpControl software	1
10903	Fluidic Unit: switching valves for various flow assays, suitable for all Perfusion Sets and channel µ-Slides, stable aluminum housing, exchangeable reservoir holders	1
10905	ibidi Pump: accuracy: +/- 1 mbar, pressure range: -100 to +100 mbar, control for up to 4 Fluidic Units, including Pump Control software	1
10908	Notebook: ready to use, pre-configured Windows system, PumpControl software	1
10991	µ-Galaxy cell culture incubator: small capacity system (14l), specially suited for setups employing up to 2 Fluidic Units, or separated setups like ECIS measurements	1
10961	Perfusion Set BLUE, length 15 cm, ID 0.8 mm, 10 ml reservoirs, designed for low flow rates. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes	3
10962	Perfusion Set RED, length 15 cm, ID 1.6 mm, 10 ml reservoirs, designed for high flow rates. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes	3
10963	Perfusion Set WHITE, length 50 cm, ID 0.8 mm, 10 ml reservoirs, designed for low flow rates and microscopy. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes	3
10964	Perfusion Set YELLOW-and-GREEN, length 50 cm, ID 1.6 mm, 10 ml reservoirs, designed for high flow rates and microscopy. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes	3
10965	Perfusion Set YELLOW, length 15 cm, ID 0.5 mm, 2 ml reservoirs, designed for low volumes and µ-Slides with 0.1 mm channel height. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes	3
10966	Perfusion Set BLACK, length 50 cm, ID 0.5 mm, 2 ml reservoirs, designed for low volumes, µ-Slides with 0.1 mm channel height, and microscopy. Ready to use tubing set for flow assays with the ibidi Pump System; tubes and connectors sterile and autoclavable; set consisting of two reservoirs and reusable silicone tubes	3
10971	Reservoir Set, 10 ml, 2 reservoirs with mounted filters, ready to use, sterile	10
10972	Reservoir Set, 2 ml, 2 reservoirs with mounted filters, ready to use, sterile	10
10973	Reservoir Set, 20 ml, 2 reservoirs with mounted filters, ready to use, sterile	10
10974	Reservoir Set, 60 ml, 2 reservoirs with mounted filters, ready to use, sterile	10
10982	ibidi Pump demo set: 1 Perfusion Set and 5 µ-Slides Luer -Type of your choice	1

Instructions

Instruction Manual ibidi Pump System (PDF)
Instruction Manual PumpControl Software (PDF)
Instruction Manual ibidi Pump System Pulsatile Flow (PDF)
Instruction Manual ibidi Pump System Oscillating Flow (PDF)

Applications:

Defined shear stress in long-term cell culture (e.g. endothelium, kidney, or biofilm)
Live cell imaging and immunofluorescence for analyzing shear stress response
Mimicking shear stress conditions in microcapillary, venous, and arterial flow
Rolling and adhesion of suspended cells on substrates
Stop flow experiments
3D cell culture: interstitial flow

Technical Features:

Operates up to four parallel Fluidic Units per ibidi Pump
Flow characteristics: all flow types are laminar
Some examples:
- Unidirectional flow simulates veins or small arteries
- Oscillating flow simulates turbulent conditions at vessel defects
- Pulsatile flow simulates arterial conditions
Flow rate: 0.03 – 35 ml/min
Shear stress: 0.3 – 150 dyn/cm²
Working volume: 2.5 / 12 ml
Suitable for all µ-Slides with Luer adapters
Also suitable for home-made flow chambers
Compatible with all incubators
Software-controlled flow rates and shear stress

System Overview:

The ibidi Perfusion System Perfectly Reflects the Natural Environment of Cells Under Flow Conditions

The ibidi Pump System consists of two main components: The ibidi Pump (computer controlled air pump) and the Fluidic Unit (holder for cell media reservoirs and μ-Slide, tubing, and electrically controlled valve set). By using this “split” approach, the closed flow setup can be assembled separately and transferred to the microscope or the incubator after cell cultivation, without compromising the sterility of the system. The open architecture using the Luer connectors allows the use of any kind of flow devices. The PumpControl software controls the pressure, and subsequently the shear stress acting on the cells. The system is a fully integrated solution.

By hosting the Fluidic Unit in the incubator, it is still possible to run perfusion assays directly on the microscope. A defined temperature and CO₂ concentration is assured, as the fluidic reservoirs are inside the incubator at all times.

Principle of Flow Generation

Applying pressured air to the reservoirs of the Fluidic Unit generates the flow in the μ-Slide channels. In order to avoid wasting any medium, the liquid is pumped back and forth from reservoir A to reservoir B by switching the four-fold valve V1.

In state 1, the pressured air is guided to reservoir A while reservoir B is connected to the ambient air pressure. In state 2, it is the other way around. Switching between these states creates a flow of medium between the reservoirs and ensures that they do not run dry. However, to generate a unidirectional flow, a fluidic rectifier is placed between the reservoirs and the μ-Slide. The rectifier is a pinch valve V2 that clamps off two branches of the Perfusion Set while the others are kept open. The synchronous switching of both valves creates a continuous and unidirectional flow without wasting any medium.

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Example of a Perfusion Set-up
on a Microscope

Ideal in combination with the ibidi Heating & Incubation System

The ibidi Pump System Generates Minimal Mechanical Stress

PumpControl Software

The PumpControl software is used to fully control the ibidi Pump System. The intuitive software allows for the easy setup of an automated flow control for flow assays. Flow rate, shear rate, and shear stress are calculated internally.

Application Examples:

rAV^CMV-LifeAct-TagRFP-transduced HUVEC, cultivated under 20 dyn / cm² in µ-Slide I^0.4 Luer

HUVEC, cultivated over 7 days at 10 dyn / cm². VE-cadherins are stained in green, cell nuclei are stained in blue.

Rolling and adhesion of polymorpho-
nuclear leukocytes on LPS-stimu-
lated cerebrovascular endothelial cells in µ-Slide VI^0.4 at 1 dyn / cm².
(Courtesy of G. Cepinskas, Ontario)

Suitable µ-Slides

The following ibidi µ-Slides can be attached to the ibidi Pump System:

Low shear stress

Medium shear stress

High shear stress

NOTE: Custom specific slides can also be used when utilizing the Luer adapters.

Perfusion Sets and µ-Slide Selection

Overview:

Blue
(ID 0.8 mm)

Red
(ID 1.6 mm)

White
(ID 0.8 mm)

Yellow
(ID 1

/Green
.6 mm)

Yellow
(ID 0.5 mm)

Black
(ID .5 mm)

Low shear stress

High shear stress

Long tubes for
microscopy (50 cm)

Working volume (ml)

2.5

Details:

	Perfusion Set blue (#10961)
	Inner diameter: 0.8 mm		Tube length: 15 cm
	Total working volume: 11.3 ml		Dead volume of tubing: 0.5 ml
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
	Growth area [cm²]	Channel volume [µl]	MIN	MAX	MIN	MAX
µ-Slide I^0.2 Luer	2.5	50	0.83	11.53	4.26	59.14
µ-Slide I^0.4 Luer	2.5	100	1.12	15.30	1.47	20.14
µ-Slide I^0.6 Luer	2.5	150	1.13	15.58	0.68	9.36
µ-Slide I^0.8 Luer	2.5	200	1.15	15.91	0.40	5.53
µ-Slide VI^0.4	0.6	30	1.12	15.25	1.97	26.86
µ-Slide y-shaped	2.8	110	1.08	14.50	2.46	32.98
without slide	-	(75)	1.18	16.51	-	-

	Perfusion Set red (#10962)
	Inner diameter: 1.6 mm		Tube length: 15 cm
	Total working volume: 12.3 ml		Dead volume of tubing: 1.5 ml
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
	Growth area [cm²]	Channel volume [µl]	MIN	MAX	MIN	MAX
µ-Slide I^0.2 Luer	2.5	50	1.28	19.11	6.57	98.02
µ-Slide I^0.4 Luer	2.5	100	2.65	33.17	3.49	43.66
µ-Slide I^0.6 Luer	2.5	150	2.74	34.98	1.65	21.02
µ-Slide I^0.8 Luer	2.5	200	2.75	35.14	0.96	12.21
µ-Slide VI^0.4	0.6	30	2.76	35.33	4.86	62.24
µ-Slide y-shaped	2.8	110	2.59	32.12	5.89	73.05
without slide	-	(75)	2.79	35.86	-	-

	Perfusion Set white (#10963)
	Inner diameter: 0.8 mm		Tube length: 50 cm
	Total working volume: 11.7 ml		Dead volume of tubing: 0.9 ml
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
	Growth area [cm²]	Channel volume [µl]	MIN	MAX	MIN	MAX
µ-Slide I^0.2 Luer	2.5	50	0.42	7.15	2.15	36.67
µ-Slide I^0.4 Luer	2.5	100	0.57	8.75	0.75	11.52
µ-Slide I^0.6 Luer	2.5	150	0.61	9.40	0.37	5.65
µ-Slide I^0.8 Luer	2.5	200	0.64	10.11	0.22	3.51
µ-Slide VI^0.4	0.6	30	0.63	9.77	1.11	17.21
µ-Slide y-shaped	2.8	110	0.58	8.94	1.32	20.33
without slide	-	(75)	0.61	9.43	-	-

	Perfusion Set yellow/green (#10964)
	Inner diameter: 1.6 mm		Tube length: 50 cm
	Total working volume: 13.6 ml		Dead volume of tubing: 2.8 ml
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
	Growth area [cm²]	Channel volume [µl]	MIN	MAX	MIN	MAX
µ-Slide I^0.2 Luer	2.5	50	1.03	17.00	5.28	87.20
µ-Slide I^0.4 Luer	2.5	100	1.91	25.95	2.51	34.16
µ-Slide I^0.6 Luer	2.5	150	1.98	27.44	1.19	16.49
µ-Slide I^0.8 Luer	2.5	200	2.13	30.25	0.47	10.51
µ-Slide VI^0.4	0.6	30	2.06	28.95	3.63	51.00
µ-Slide y-shaped	2.8	110	1.94	26.60	4.41	60.50
without slide	-	(75)	2.44	36.02	-	-

	Perfusion Set yellow (#10965)*
	Inner diameter: 0.5 mm		Tube length: 15 cm
	Total working volume: 2.5 ml		Dead volume of tubing: 0.5 ml
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I^0.1 Luer	2.5	25	0.07	1.35	1.42	27.34
µ-Slide III^0.1	0.43	4.5	0.06	1.08	6.40	115.27
µ-Slide VI^0.1	0.17	1.7	0.08	1.44	8.54	153.69

* Perfusion Set only works with PumpControl version 1.5.0 or higher and
ibidi Pump with hardware version 1.02 or higher and firmware version 1.10 or higher.

	Perfusion Set black (#10966)*
	Inner diameter: 0.5 mm		Tube length: 50 cm
	Total working volume: 2.7 ml		Dead volume of tubing: 0.7 ml
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I^0.1 Luer	2.5	25	0.03	0.61	0.61	12.36
µ-Slide III^0.1	0.43	4.5	0.03	0.52	3.20	55.50
µ-Slide VI^0.1	0.17	1.7	0.03	0.62	3.20	66.17

* Perfusion Set only works with PumpControl version 1.5.0 or higher and
ibidi Pump with hardware version 1.02 or higher and firmware version 1.10 or higher.