• ibidi Heating System, Universal Fit
  • ibidi Heating System, Universal Fit

ibidi Heating System, Universal Fit

Heating System for all live cell imaging applications including high resolution, TIRF, and confocal microscopy

  • Compatible with all microscopy platforms - fits every inverted microscope that has a frame or holder for 96 well plates
  • Excellent illumination of the sample - no condensation due to heated lid, optimal magnetic sample stabilization, and suitable for DIC
  • Perfectly suited for live cell imaging applications - full incubator conditions on the microscope
  • Ideal for use with motorized stages when used with the Heated Plate for 4 µ-Slides

ibidi Temperature Controller

+

Heated Plate in
Multi-Well Format

+

Heated Glass Lid,
Universal Fit

+

Heating Insert for ibidi
and non-ibidi Slide Formats


Contact ibidi for a free demo of the ibidi Heating & Incubation System.

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Cat. No. Description Pcs./Box
10918 ibidi Heating System, Universal Fit, for 1 Chamber: ibidi Temperature Controller, Heated Plate in Multi-Well Format for 1 Chamber, 1 Heating Insert, with Heated Lid 1
10915 ibidi Heating System, Universal Fit, for 1 Chamber: ibidi Temperature Controller, Heated Plate in Multi-Well Format for 1 Chamber, 1 Heating Insert, without Heated Lid 1
10927 ibidi Heating System, Universal Fit, for 4 µ-Slides: ibidi Temperature Controller, Heated Plate in Multi-Well Format for 4 µ-Slides, with Heated Lid 1
10928 Heated Plate, Universal Fit, for 4 µ-Slides: Heated Plate in Multi-Well Format for 4 µ-Slides 1
10932 Heating Insert µ-Dish 35 mm, low for ibidi Heating System, Universal Fit: insert for µ-Dish 35 mm, low 1
10933 Heating Insert µ-Slide for ibidi Heating System, Universal Fit: insert for all ibidi µ-Slides 1
10934 Heating Insert µ-Dish 35 mm, high for ibidi Heating System, Universal Fit: insert for µ-Dish 35 mm, high 1
10937 Heating Insert LabTek for ibidi Heating System, Universal Fit: insert for LabtekTM chambered coverglass 1
10936 Heating Insert Adapter for perfusion assays: for ibidi Heating System, Universal Fit, for 1 Chamber 1
11930 Modification Heated Lid ibidi Heating System, Universal Fit, for Perfusion Assays, Type A: 2 holes on transverse side 1
11931 Modification Heated Lid ibidi Heating System, Universal Fit, for Perfusion Assays, Type B: 6 holes on each long side 1
11932 Modification Heated Lid ibidi Heating System, Universal Fit, for Perfusion Assays, Type C: customized 1

Applications:

 

 

 

Specifications:

Temperature Controller
Environmental humidity Relative humidity
0% - 99%
Environmental
temperature
+5°C - +40°C
(50% rel. humidity)
Dimensions
(H x W x D in mm)
90 x 170 x 230
Weight 3.4 kg
Input voltage 230 V / 50 Hz
115 V / 60 Hz
 

 

Heated Plate and Lid
Dimensions Plate / Lid
(in mm)
127.5 x 85.5 x 8 /
135 x 85.5 x 20.5
Height of the system 26.5mm
Weight of incubation chamber with insert 330 g
Gas inlet Luer connector (M5 thread) with Luer Lock
Restrictions for objective lenses
None
Custom openings On request


Heating Stages for Live Cell Imaging

The independently controlled, heated glass lid of the ibidi Heating & Incubation System solves the problem of condensation in live cell imaging.

By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.

 


click here to enlarge


Components of the ibidi Heating & Incubation System, Universal Fit

ibidi Temperature Controller

  • Provides excellent temperature stability within the incubation chamber
  • Four control channels allow to connect a heated plate, a heated lid, and two additional heated components (e.g., an objective heater)

Technical Features:

  • Temperature stability: ± 0.05 °C
  • Temperature uniformity across the insert: ± 0.5 °C
  • Temperature range: Room temperature - 45 °C
  • Control channels: 4
  • TempControl software for remote control and data recording included
  • External temperature sensor included
  • Adjustable alarm function included
  • Analog output for extreme noise reduction optional

Heated Plate in a Multi-well Format for 1 Chamber

  • Can be used with any inverted microscopy system
  • Fits into both manual and automated stages
  • Can be placed into any regular multi-well frame that comes with your microscope
  • Adaptable to all types of microscopy chambers, such as µ-Slides and µ-Dishes plus non-ibidi formats

Heated Plate in a Multi-well Format for 4 µ-Slides

  • Can be used with any inverted microscopy system
  • Ideal for use with motorized stages
  • Can be placed into any regular multi-well frame that comes with your microscope
  • Compatibe with all ibidi µ-Slides and non-ibidi slide formats

Heated Glass Lid, Universal Fit, for Use with CO2 and Humidity

  • For use with CO2, O2, and humidity to provide a complete environmental chamber
  • Independently controlled glass lid, warmed to 40 °C, prevents the formation of condensed water on the lid of the microscopy chamber or plate
  • No scattering of light or shadow formation

Heating Inserts

Heating Insert Adapter for Perfusion Assays

  • To be used with the ibidi Pump System for live cell imaging under flow conditions 

Gas Mixer for the Incubation Unit

The heated lid also comes with a gas inlet. The ibidi Gas Incubation System upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications, including high resolution, TIRF, and confocal microscopy.

The ibidi Gas Incubation System is suitable for various experimental conditions (e.g., pH or hypoxia). It is available in two versions: Gas Mixer for CO2, Gas Mixer for CO2 and O2.


Live Cell Imaging Under Flow Conditions

The ibidi Heating & Incubation System can be adapted for live cell imaging under flow conditions. Connect the ibidi Pump System with the ibidi Heating System, Universal Fit, by using the Heating Insert Adapter for perfusion assays. This creates the perfect environment for long-term cell studies (several days) under flow conditions.


Click to enlarge



Overview of the Heating and Incubation System, Universal Fit


ibidi Heating System for Temperature Control
of Multi-Well Plates

ibidi has developed a Heating System for the use with K-Frame Stages or the Nikon Ti-S-E and Ti-S-ER Motorized Stage which hold multi-well plates. Thus, this system permits the user to control the temperature of samples held in multi-well plates.


Special Solutions

You can also obtain additional customer-specific solutions upon request.

 



Tim Sanchez, Harvard University, Cambridge, USA

"For my research, I need to take time-lapse images of mouse embryos developing from the 1 cell stage all the way to 5-day blastocyst. Anyone who has worked with mouse embryos knows they are incredibly sensitive and delicate, so we required an extremely stable environment over several days. I struggled for months with a different system, but the ibidi Heating Stage worked very quickly. I was immediately impressed with the quality. Calibration with the included thermocouple was easy and accurate. Now I get consistent development every time, and the percentage of embryos developing to blastocysts is the same as the control incubator! Terrific product, thanks ibidi.”

Tim Sanchez, Harvard University, Cambridge, USA
http://www.needleman.seas.harvard.edu/index.html


Loïc Dupré, INSERM, Toulouse, France

"The ibidi µ-Slides Chemotaxis 2D and 3D have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.

I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"

Loïc Dupré PhD, INSERM UMR 1043, Purpan University Hospital, Toulouse, France
http://www.cptp.inserm.fr/


Prof. Dr. Stefan Zahler, University of Munich, Germany

„Using the „angiogenesis slide“ from ibidi is the only possibility that I know for achieving a consistently good optical quality in the tube formation assay, and saving Matrigel at the same time.

In comparison to other systems, the heating stage from ibidi shows superior thermal stability and enables you to work with high humidity (>80%). This is not possible with other systems but is indispensable for long-term studies.“

Prof. Dr. Stefan Zahler, Pharmaceutical Biology, Munich Center for System-Based Drug Research, Ludwig-Maximilians-University, Germany
www.pharmbiol.cup.uni-muenchen.de

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