• ibidi Gas Incubation System
  • ibidi Gas Incubation System

ibidi Gas Incubation System

A gas mixer that upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications with CO2, O2, and humidity control

  • Ideal for live cell imaging applications - full incubator conditions on the microscope
  • Stable gas incubation without evaporation (patent pending technology)
  • Suitable for various experimental conditions (e.g., pH or hypoxia)
  • Fully compatible with the ibidi Heating Systems  and stage top incubators of other suppliers


ibidi Gas Mixer

+

Humidifying
Column


Optional:
Air Pressure Generator


Contact ibidi for a free demo of the ibidi Heating & Incubation System.


 

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Cat. No. Description Pcs./Box
11920 ibidi Gas Incubation System for CO2: ibidi CO2 Gas Mixer, Humidifying Column 1
11922 ibidi Gas Incubation System for CO2 and O2: ibidi CO2 and O2 Gas Mixer, Humidifying Column 1
11929 Air Pressure Generator, 1 bar, ready to use with the ibidi Gas Incubation System 1

Applications:


Technical Features:

  • Precise and reliable gas incubation for O2 and CO2
  • Active and fast humidification - no evaporation
  • Uses pressurized air to create the gas flow – no vibrations
  • Optional air pressure generator available (for when pressurized air is not obtainable)
 

The ibidi Gas Incubation System provides both humid and CO2-rich air for stage-top incubators like the ibidi Heating System. The gas mixture is continuously flushed through the stage top incubator, ensuring a maximum humidity and an optimal pH for CO2-buffered liquids.

Spheroid of breast cancer cells with living cells (green) and apoptotic center (red) due to oxygen depletion.


Components of the ibidi Gas Incubation System

ibidi Gas Mixer for CO2 and O2

  • Precise and reliable gas incubation
  • Active and fast humidification without evaporation (patent pending technology)
  • Suitable for various experimental conditions (e.g., pH or hypoxia)
  • Fully compatible with the ibidi Heating Systems
  • Uses pressurized air to create the gas flow – no vibrations
  • Optional air pressure generator available (for when pressurized air is not obtainable)

Humidifying Column

  • Part of the ibidi Gas Incubation System
  • Humidifies the outgoing CO2 / O2 / air mix to minimize sample evaporation
  • Active humidity control inside the attached ibidi Heating System


The ibidi Gas Incubation System upgrades your ibidi Heating System to a complete stage top incubator.

Location/Tissue Oxygen Partial Pressure p(O2) Corresponding O2 in
Cell Culture Gas Mix (%)
Inspired air 21 kPa (=160 mm Hg) 21%
Alveolus 14 kPa (=104 mm Hg) 14%
Arterial blood 14 kPa (=104 mm Hg) 14%
Veneous blood 5 kPa (=40 mm Hg) 5%
Interstitial fluid 5 kPa (=40 mm Hg) 5%
Healthy tissue 2-3 kPa (=15- 23 mm Hg) 2-3%
Tumor without blood vessels  
   Outside 1 kPa (=7.5 mm Hg) 1%
   Center 0 kPa (=0 mm Hg) 0%
Normoxia conditions 2 - 11 kPa (=15 - 82 mm Hg) 2-11%
Hypoxia conditions 0 - 1 kPa (=0 - 7.5 mm Hg) 0-1%
Cell culture incubator (5% CO2) 20 kPa (=150 mm Hg) 20%

Overview of the ibidi Heating & Incubation System, CO2 and O2 Control

Gas Supply (Input)

Specifications:

Gas Supply (Input)
Pressurized Air 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
CO2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
N2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)

Tubing for all
gasses (Supplied with the system)
PUR/PUN tubing
4 mm inner diameter
6 mm outer diameter
1 mm wall thickness
 

 



ibidi Gas Mixer

Specifications:

CO2 Control  
CO2 Control Range 0.1% - 20%
Accuracy 0.1% - 0.5% (absolute)
   
O2 Control  
O2 Control Range 0% - 21%
Accuracy 0.5 % (absolute)
   
Gas Flow  
Control Range 5 - 20 l/h


Humidifying Column

Specifications:

Humidity Control
Control Range 20% - 99% (Rel. Humidity)
Accuracy 1% (absolute)
Water Refill Every 7 days


ibidi Heating System

Compatible with:

 





Loïc Dupré, INSERM, Toulouse, France

"The ibidi µ-Slides Chemotaxis 2D and 3D have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.

I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"

Loïc Dupré PhD
INSERM UMR 1043
Purpan University Hospital
Toulouse
France

http://www.cptp.inserm.fr/

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