• Nanolive-ibidi Gas Incubation System
  • Nanolive-ibidi Gas Incubation System

Nanolive-ibidi Gas Incubation System

A gas mixer that upgrades the Nanolive-ibidi Heating System to a complete stage top incubator for all live cell imaging applications with CO2, O2, and humidity control on the 3D Cell Explorer

in cooperation with

Nanolive-ibidi Gas Mixer

+

Humidifying
Column


Optional:
Air Pressure Generator


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Cat. No. Description Pcs./Box
11920-N Nanolive-ibidi Gas Incubation System for CO2: Nanolive-ibidi CO2 Gas Mixer, Humidifying Column 1
11922-N Nanolive-ibidi Gas Incubation System for CO2 and O2: Nanolive-ibidi CO2 and O2 Gas Mixer, Humidifying Column 1
11929-N Air Pressure Generator, 1 bar, ready to use with the Nanolive-ibidi Gas Incubation System 1

Instructions

Instruction Manual Nanolive-ibidi Gas Incubation System for CO2 and O2 (PDF)
Instruction Manual Nanolive-ibidi Temperature Controller (PDF)

Product Flyer

3D CELL EXPLORER - A revolutionary Tomographic Microscope to instantly look inside Living Cells in 3D
Detailed Information (PDF)
Short Information (PDF)

Applications:

  • Single cell morphology, kinetics, and dynamics (e.g., cell death, remodeling, migration, adhesion, or cellular tracking)
  • Cellular interactions and reactions (e.g., drugs, viruses, bacteria, or nanomaterials)
  • Stain-free histology and histopathology (e.g., tissues morphological analysis, 3D tissue characterization, or cancer diagnosis)
  • Stain-free cytopathology
  • Cell’s metrics


Technical Features:

  • Precise and reliable gas incubation for O2 and CO2
  • Active and fast humidification - no evaporation
  • Uses pressurized air to create the gas flow – no vibrations
  • Optional air pressure generator available (for when pressurized air is not obtainable
 

The Nanolive-ibidi Gas Incubation System provides both humid and CO2-rich air for stage-top incubators like theNanolive-ibidi Heating System. The gas mixture is continuously flushed through the stage top incubator, ensuring a maximum humidity and an optimal pH for CO2-buffered liquids.


Components of the Nanolive-ibidi Gas Incubation System

Nanolive-ibidi Gas Mixer for CO2 and O2

  • Precise and reliable gas incubation
  • Active and fast humidification without evaporation (patent pending technology)
  • Suitable for various experimental conditions (e.g., pH or hypoxia)
  • Fully compatible with the Nanolive-ibidi Heating Systems
  • Uses pressurized air to create the gas flow – no vibrations
  • Optional air pressure generator available (for when pressurized air is not obtainable)

Humidifying Column

  • Part of the Nanolive-ibidi Gas Incubation System
  • Humidifies the outgoing CO2 / O2 / air mix to minimize sample evaporation
  • Active humidity control inside the attached Nanolive-ibidi Heating System


The Nanolive-ibidi Gas Incubation System upgrades your Nanolive-ibidi Heating System to a complete stage top incubator.

Location/Tissue Oxygen Partial Pressure p(O2) Corresponding O2 in
Cell Culture Gas Mix (%)
Inspired air 21 kPa (=160 mm Hg) 21%
Alveolus 14 kPa (=104 mm Hg) 14%
Arterial blood 14 kPa (=104 mm Hg) 14%
Veneous blood 5 kPa (=40 mm Hg) 5%
Interstitial fluid 5 kPa (=40 mm Hg) 5%
Healthy tissue 2-3 kPa (=15- 23 mm Hg) 2-3%
Tumor without blood vessels  
   Outside 1 kPa (=7.5 mm Hg) 1%
   Center 0 kPa (=0 mm Hg) 0%
Normoxia conditions 2 - 11 kPa (=15 - 82 mm Hg) 2-11%
Hypoxia conditions 0 - 1 kPa (=0 - 7.5 mm Hg) 0-1%
Cell culture incubator (5% CO2) 20 kPa (=150 mm Hg) 20%

Overview of the Nanolive-ibidi Heating & Incubation System, CO2 and O2 Control

Gas Supply (Input)

Specifications:

Gas Supply (Input)
Pressurized Air 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
CO2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
N2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)

Tubing for all
gasses (Supplied with the system)
PUR/PUN tubing
4 mm inner diameter
6 mm outer diameter
1 mm wall thickness
 

 



Nanolive-ibidi Gas Mixer

Specifications:

CO2 Control  
CO2 Control Range 0.1% - 20%
Accuracy 0.1% - 0.5% (absolute)
   
O2 Control  
O2 Control Range 0% - 21%
Accuracy 0.5 % (absolute)
   
Gas Flow  
Control Range 5 - 20 l/h


Humidifying Column

Specifications:

Humidity Control
Control Range 20% - 99% (Rel. Humidity)
Accuracy 1% (absolute)
Water Refill Every 7 days


Nanolive-ibidi Heating System

For use with ibidi µ-Dishes and µ-Slides and non-ibidi formats

 





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