• ECIS System
  • ECIS System

ECIS System

A morphological biosensor used to measure cell behavior which employs a label-free, non-invasive method to electronically monitor cells grown in tissue culture

  • Non-invasive cell-based assays in culture medium
  • Measurement of changes in cell morphology
  • Multiple, real-time measurements

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Cat. No. Description Pcs./Box
71616 ECIS Model Z: ECIS Model Z station controller; Elevated Field Module (i.e., a pulse system for automated wound healing or electroporation studies); software for data gathering and analysis; laptop 1
71617 ECIS Model Z Theta: ECIS Model Z Theta station controller; Elevated Field Module (i.e., a pulse system for automated wound healing or electroporation studies); software for data gathering and analysis, including model calculations; laptop 1
71612 16 well station: A holder with two slots for ECIS Cultureware 8 well arrays, and a data processing station 1
71614 96 well station: A holder with one slot for ECIS Cultureware 96 well arrays, and a data processing station 1
71621 Upgrade 96 well station: to bed of nails on 96 well Array Station 1
71001 ECIS Flow Module perfusion system for ECIS flow arrays, ideal for defined flow rates and shear stress studies of endothelial cells 1
71609 ECIS Transwell Measurement System: Permanent transwell holder to run TEER measurements. Made for filter-based assays. Contains a lower Filter holder base and an upper counter electrode system, reusable 1

Applications:

 

Cells grown on an ECIS electrode

The ECIS method represents a non-invasive approach to monitor living cells in vitro. It measures the change in impedance of a small electrode over time.

In this system, cells are grown on small gold film electrodes carrying very weak AC signals. As cells attach and spread on these electrodes, their insulating membranes block and constrain the current flow, resulting in measured variations in the electrode impedance. Any changes in the environment, leading to morphological changes of the cell, alter the current paths and can be readily detected by the instrumentation. These changes may be due to the addition of compounds, changes in the physical environment (for example perfusion, transfection events, viral infections, and the addition of other cells).

The ECIS system gathers continuous real-time measurements without the need for any labels. Furthermore, since the weak AC currents used during measurement have no effect upon the cells, this method is totally non-invasive.

There are two major systems: The ECIS Model Z and the ECIS Model Zθ (theta). The ECIS Model Z system monitors the impedance of small 250-micrometer diameter electrodes used as substrates for cell growth. The ECIS Model Zθ system interprets complex impedance as both resistance and capacitance, and can report these values as well as simple impedance. This data can then be further refined using a model that provides information on the barrier function of cell layers (Rb), the spacing beneath the cells (α), and capacitance of the cell membrane (Cm).

Both systems are available with either a 2 x 8 well or a 96 well array station that is ideal for high throughput applications. The array stations are hosted in an incubator so that cells are kept under ideal cultivation conditions at all times.


Specifications for ECIS Z and ECIS Zθ

See: ECIS Instrument Selection Guide 

Basic Principle of ECIS Measurement


ECIS Cultureware

The ECIS Cultureware consists of disposable electrode arrays containing gold film electrodes. Click here to learn more about the various types of ECIS Cultureware.

For further information please contact us.

For more than 800 scientific publications using the ECIS technology please visit www.biophysics.com.


Impedance Measurements Under Flow Conditions

For impedance measurements under flow conditions, combine the ECIS System with the Pump System to more closely simulate the in vivo environment.


 

 


ECIS Transwell Measurement System

This system is designed to measure the TEER (Trans Epithelial / Endothelial Electric Resistance) of cell layers grown on filter inserts. 

  • Easily comparable to classic TEER measurements
  • Ideal fo addressing lung epithelial cell cultures 

The ECIS Transwell Measurement System is a permament transwell holder to measure the TEER of cell layers. The holder contains a lower Filter holder base and an upper counter electrode system. It is used in filter-based assays.

 


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