A morphological biosensor used to measure cell behavior which employs a label-free, non-invasive method to electronically monitor cells grown in tissue culture
- Non-invasive cell-based assays in culture medium
- Measurement of changes in cell morphology
- Multiple, real-time measurements
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|71616||ECIS Model Z: ECIS Model Z station controller; Elevated Field Module (i.e., a pulse system for automated wound healing or electroporation studies); software for data gathering and analysis; laptop||1|
|71617||ECIS Model Z Theta: ECIS Model Z Theta station controller; Elevated Field Module (i.e., a pulse system for automated wound healing or electroporation studies); software for data gathering and analysis, including model calculations for cell barrier function; laptop||1|
|71612||16 well station: A holder with two slots for ECIS Cultureware 8 well arrays, and a data processing station||1|
|71614||96 well station: A holder with one slot for ECIS Cultureware 96 well arrays, and a data processing station||1|
|71621||Upgrade 96 well station: to bed of nails on 96 well Array Station||1|
|71001||ECIS Flow Module perfusion system for ECIS flow arrays, ideal for defined flow rates and shear stress studies of endothelial cells||1|
In this system, cells are grown on small gold film electrodes carrying very weak AC signals. As cells attach and spread on these electrodes, their insulating membranes block and constrain the current flow, resulting in measured variations in the electrode impedance. Any changes in the environment, leading to morphological changes of the cell, alter the current paths and can be readily detected by the instrumentation. These changes may be due to the addition of compounds, changes in the physical environment (for example perfusion, transfection events, viral infections, and the addition of other cells).
Cells grown on an ECIS electrode
Basic Principle of ECIS Measurement
There are two major systems: The ECIS Model Z and the ECIS Model Zθ (theta). The ECIS Model Z system monitors the impedance of small 250-micrometer diameter electrodes used as substrates for cell growth. The ECIS Model Zθ system interprets complex impedance as both resistance and capacitance, and can report these values as well as simple impedance. This data can then be further refined using a model that provides information on the barrier function of cell layers (Rb), the spacing beneath the cells (α), and capacitance of the cell membrane (Cm).
Both systems are available with either a 2 x 8 well or a 96 well array station that is ideal for high throughput applications. The array stations are hosted in an incubator so that cells are kept under ideal cultivation conditions at all times.