• ECIS Cultureware
  • ECIS Cultureware

ECIS Cultureware

The ECIS Cultureware consists of sterile disposable electrode arrays containing gold film electrodes. The gold layer is thin enough to allow microscopic observation of the cells using a standard inverted tissue culture microscope. The ECIS electrode array is placed in an array holder located in the incubator.


The cultureware includes:

  • Standard 8 well arrays
  • Standard 96 well arrays
  • Specialty arrays (e.g., flow, chemotaxis)

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Cat. No. Description Pcs./Box
72001 ECIS Cultureware 8W1E PET: 8 well array with 1 electrode per well, ideal for wound healing and migration studies, transparent PET bottom 6
72002 ECIS Cultureware 8W2X1E, Medusa PET: 8 well array with 2 individual electrodes per well, ideal for reference measurements, transparent PET bottom 6
72005 ECIS Cultureware 8W2LE PET: 8 well line wounding array with a single 667 µm x 150 µm linear electrode, ideal for wound healing and migration studies, transparent PET bottom 6
72010 ECIS Cultureware 8W10E PET: 8 well array with 10 electrodes per well, ideal for adhesion studies and other cellular assays, transparent PET bottom 6
72021 ECIS Cultureware 8W1LE PET: 8 well chemotaxis array with 1 electrode per well, used for monitoring the movement of cells in response to chemical gradients, transparent PET bottom 6
72030 ECIS Cultureware 8WCP PET: 8 well array with rectangular electrodes on inter-digitated fingers sampling over the well’s entire bottom, ideal for cell proliferation assays, transparent PET bottom 6
72040 ECIS Cultureware 8W10E+ PET: 8 well array with 40 electrodes per well, ideal for adhesion studies and other cellular assays, transparent PET bottom 6
72096 ECIS Cultureware 96W1E+ PET: 96 well array with 2 electrodes per well, ideal for wound healing and migration studies, transparent PET bottom 6
72097 ECIS Cultureware 96W10idf PET: 96 well array with 10 electrodes per well, ideal for adhesion studies and other cellular assays, transparent PET bottom 6
72098 ECIS Cultureware 96W20idf PET: 96 well array with 20 electrodes per well, ideal for adhesion studies and other cellular assays, transparent PET bottom 6
70101 ECIS Flow array 1E channel µ-Slide with 8 x 1 electrodes: specially designed for endothelial cell studies and cell - cell interaction at defined flow rates (ca. 0.35 mm channel height, other channel heights available on request) 6
70110 ECIS Flow array 10E channel µ-Slide with 8 x 10 electrodes: specially designed for endothelial cell studies and cell - cell interaction at defined flow rates (ca. 0,35 mm channel height, other channel heights available on request) 6

Substrates for ECIS Cultureware

Applied Biophysics offers two different substrates for the ECIS cultureware. The classic Lexan substrate offers superb imaging quality through the thin gold layer. It gives best results if ECIS measurements are combined with microscopy.

In 2013, Applied Biophysics has developed a substrate, made out of PET, for all arrays. In these arrays, the gold electrode is located on 254 µm thick PET material and is optically clear, so that cells can be viewed on an inverted microscope.This base material has become the new standard. With the PET substrate, Applied Biophysics is offering an economic alternative to the already existing Lexan substrates.

If you require Lexan arrays, please contact us at info@ibidi.de.

Types of ECIS Cultureware

   

8W1E

Each of the 8 wells contains a single circular 250 µm diameter measuring electrode*.

Applications include:

 


8W10E

Each of the 8 wells contains ten 250 µm diameter measuring electrodes connected in parallel on a common gold pad*.

Applications include:

  • Recording the activities of more cells over a larger area of the substrate
  • Studying the effects of agents upon overall impedance
  • Reducing fluctuations in impedance due to micromotion that may interfere with some assays
 


8W10E+

Each of the 8 wells contains forty 250 µm diameter measuring electrodes connected in parallel on a common gold pad*. The array records the activities of more cells over a larger area of the substrate. Fluctuations in impedance are reduced, thus micromotion might not be observed.

Applications include:

 


8W1E DD

This array is nearly identical to the 8W1E standard array but the active electrodes* come in four different diameters (two wells of each size). The diameters are 250 (standard), 100, 50 and 25 µm.

Application:

  • Monitoring fewer cells with higher sensitivity
 


8W2x1E (Medusa)

This array contains 8 individual wells, with each well having two independent standard measuring electrodes*. This allows researchers to perform duplicate measurements within a single well. When connected to the array holder, only the upper four wells are measured. To use the other four wells, the array is turned around and the contact pads at the other end are connected.

Applications include:

 


8WCP

Each of the 8 wells contains two sets of four 7.5 mm x 0.5 mm rectangular electrodes located on inter-digitated fingers so as to provide cell measurements upon a total of 8 large electrodes.

Application:

  • Cell proliferation assays
 


8W2LE

Each of the 8 wells contains a single 667 µm x 150 µm linear electrode whose area is equal to that of our standard 250 µm circular electrodes.

Applications include:

 


8W1LE ChemoTaxis

This array is used in chemotaxis measurements as first described by Hadjout, N. et al. in Biotechniques, 31 (5): 1130-1138 (2001) . The measuring electrode in this array is a thin gold line between two registry marks*.
 


96W1E+ | 96W10idf | 96W20idf  

Each of the 96 wells in a standard plate configuration contains two circular 350 µm diameter measuring electrodes (96W1E+) or has an inter-digitated finger configuration (96W10idf, 96W20idf). These arrays have been designed for high throughput assays. The exceptional signal-to-noise ratio allows studies of very sparse cultures, even single cells.

Applications include:

 




ECIS Flow Array 1E | 10E

A specialized array with 8 active ECIS electrodes* located in the central region at the base of a flow channel (50 mm in length, 5 mm in width, around 0.35 mm in height). Flow characteristics are comparable to the µ-Slide I 0.4 Luer.

Applications include:

Please note: The basis for the ECIS Flow array is an ibidi µ-Slide 0.1 Luer. The indicated height of approximately 350 µm is the sum of 100 µm channel height and a 250 µm layer of glue. For other channel heights, please contact us.

 


Customize your ECIS arrays     

If you are interested in other electrode sizes and configurations, please contact us – we will be happy to work with you on your specific needs.

 



All arrays can be ordered without upper chambers (NC).

+ The economic PCB arrays are opaque and therefore not accessible to microscopy.
* Gold films are transparent and the upper chambers can be removed after ECIS measurements to facilitate immunofluorescence staining. 
   
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