µ-Proteofection Kit

For the direct insertion of proteins (e.g., antibodies) into the cytosol of living cells

Cell culture, proteofection, and microscopy – all in one slide
Reagents optimized for proteins or antibodies, respectively
Fast and simple protocol

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Cat. No.	Description	Pcs./Box
60641	µ-Proteofection Kit VI, for direct transport of proteins through the cell membrane, 15 µ-Slides VI 0.4, ibiTreat, sterile, 250 µl PROTEOfectene®, 100 µg/ml R-Phycoerythrin in 100 µl PBS	1
60642	µ-Proteofection Kit VI AB, for direct transport of antibodies through the cell membrane, 15 µ-Slides VI 0.4, ibiTreat, sterile, 250 µl PROTEOfectene® AB, 100 µg/ml FITC-IgG in 100 µl PBS	1

Applications:

Protein localization through fluorescent antibodies
Intracellular competitive protein inhibition
Protein studies in knock down organisms

Technical Features:

90 proteofection experiments per kit
PROTEOfectene^® optimized for protein insertion
PROTEOfectene^® AB optimized for antibody insertion
The kit contains PROTEOfectene^® (AB), appropriate positive control reagents, and µ-Slide VI^0.4

Specifications / Content:

µ-Slide (ibidi)	µ-Slide VI^0.4, ibiTreat (15x)
Reagent (Biontex)	PROTEOfectene^® (250 µl)
Positive control (Biontex)	R-Phycoerythrin, 100 µg/ml in PBS (100 µl)

µ-Slide (ibidi)	µ-Slide VI^0.4, ibiTreat (15x)
Reagent (Biontex)	PROTEOfectene^®AB (250 µl)
Positive control (Biontex)	FITC-IgG, 100 µg/ml in PBS (100 µl)

The PROTEOfectene^® reagents (Biontex Laboratories GmbH) were developed to enable the direct uptake of proteins and antibodies through the lipid membrane of cells by endocytosis. Using this procedure, shuttled proteins reach the cytosol and act directly in signal transduction. If the antibodies/proteins are labeled, their fate can be followed directly using microscopy.

To ensure that the proteofection procedure is working, either a fluorescent protein (R-Phycoerythrin) or an antibody (FITC-IgG) is included in the kit. These positive controls are useful tools for an easy evaluation of the experimental results.