• CPOx - Extracellular O<sub>2</sub> Monitoring
  • CPOx - Extracellular O<sub>2</sub> Monitoring

CPOx - Extracellular O2 Monitoring

Fluorescence lifetime probes for quantitative extracellular O2 measurement (to be used with the ibidi OPAL System and FLIM microscopes)

  • Exact oxygen measurement in cell cultures or tissue
  • Fast measurement within seconds
  • High spatial resolution
  • Non-invasive and real-time measurements


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Cat. No. Description Pcs./Box
74101 CPOx-Beads, red: fluorescence lifetime probe for extracellular O2 measurement, Ø 50 µm, 3 mg, 5 mg/ml, (10-100 assays) 1
74102 CPOx-Beads, red: fluorescence lifetime probe for extracellular O2 measurement, Ø 50 µm, 10 mg, 5 mg/ml (30-300 assays) 1
74111 CPOx-Beads, orange: fluorescence lifetime probe for extracellular O2 measurement, Ø 50 µm, 3 mg, 5 mg/ml (10-100 assays) 1
74112 CPOx-Beads, orange: fluorescence lifetime probe for extracellular O2 measurement, Ø 50 µm, 10 mg, 5 mg/ml (30-300 assays) 1

Applications:

  • Oxygen measurement in cell cultures and tissues
  • Ideal for in vitro hypoxia conditions, like 3D cell cultures, spheroid models, and tissue


Oxygen monitoring is defined as the measurements of O2 in the direct neighborhood of cultured cells. Oxygen sensitive beads, with a diameter of 50 µm (CPOx), are used in combination with the ibidi OPAL system or a FLIM microscope to measure the oxygen concentration.

 

Technical Features:

  • 50 µm polystyrene beads with an O2-sensitive fluorophore (fluorescence lifetime)
  • Quantitative results with OPAL and FLIM microscopes
  • Biocompatible in cell culture, spheroids,
    or tissue
  • Not cell permeable
  • For extracellular O2 monitoring
 

 Specifications:


CPOx orange CPOx red
Composition 50 µm beads 50 µm beads
Ex 530 nm
(510-550 nm)
540 nm
(500-550 nm)
Em 600 nm
(570-670 nm)
650 nm
(640-670 nm)
Lifetime (37°C) 4.0 µs (21% O2)
5.3 µs (0% O2)
20 µs (21% O2)
57 µs (0% O2)


Overview: O2 Monitoring and O2 Imaging

ibidi OPAL
on Fluorescence Microscope

(Phosphorescence Lifetime Measurement)
CPOx Beads
NanO2
Quantitative
Extracellular
Not Image-Based
Quantitative
Intracellular
Not Image-Based
PLIM Microscope
(Phosphorescence Lifetime Imaging):
 
CPOx Beads
NanO2
Quantitative
Extracellular
Image-Based
Quantitative
Intracellular
Image-Based
Fluorescence Microscope
(Intensity-Based Imaging):
 
MM2
Quantitative
Intracellular
Image-Based


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