• LifeAct Plasmids
  • LifeAct Plasmids

LifeAct Plasmids

An actin marker for the visualization of F-actin in living cells after plasmid transfection

  • Brilliant visualization of F-actin in living cells - perfect imaging of cytoskeletal organization and cellular dynamics
  • No interference with cytoskeletal dynamics - unrestricted actin functionality
  • Excellent signal-to-noise ratio
  • Long-term actin staining with stable cell lines

For pricing and online ordering, please log in.

Cat. No. Description Pcs./Box
60101 Plasmid pCMVLifeAct-TagGFP2, F-actin marker for transfection, ready to use, 20 µg, concentration 500 ng/µl 1
60102 Plasmid pCMVLifeAct-TagRFP, F-actin marker for transfection, ready to use, 20 µg, concentration 500 ng/µl 1
60106 Plasmid pCAGLifeAct-TagGFP2, F-actin marker for transfection, ready to use, 20 µg, concentration 500 ng/µl 1
60107 Plasmid pCAGLifeAct-TagRFP, F-actin marker for transfection, ready to use, 20 µg, concentration 500 ng/µl 1

Applications:

  • Cytoskeleton organization:
    -  Non-toxic actin visualization 
    -  Fluorescence staining
  • Cytoskeletal dynamics:
    -  Actin dynamics in cellular processes
    Live cell imaging and microscopy


Watch our Movie:
Live Cell Imaging of Actin Dynamics in Primary Dendritic Mouse Cells, Transfected with pCMVLifeAct-TagGFP2


Technical Features:

  • For transient and stable  transfections
  • Superb biocompatibility - non-toxic staining of living samples
 

Specifications:

Concentration 500 ng / μl
Storage -20 °C
TagGFP2 (Exmax / Emmax) 483 / 506 nm
TagRFP (Exmax / Emmax) 555 / 584 nm




LifeAct, a 17-amino acid peptide, is derived from a protein from Saccharomyces cerevisiae. LifeAct stains filamentous actin (F-actin) structures in living or fixed eukaryotic cells and tissues. In contrast to GFP-actin and its alternatives such as actin-binding proteins, LifeAct does not interfere with actin dynamics in vitro and in vivo, as shown in several cell types and model organisms.*

LifeAct-Plasmids may be used in many cell types when a transient or stable expression of LifeAct is needed. In this case, researchers can choose between two different promoters, CMV (cytomegalovirus) or CAG (modified chicken β-actin). For many standard cell lines, the CMV promoter is the best choice as it leads to strong expression of LifeAct. However, when working with sensitive cells such as neuronal or embryonic stem cells, it is most often recommended to use the CAG promoter, because it does not have a viral origin and an interference with cellular defense mechanisms (“silencing mechanisms”) is less likely.

 



After transfection of cells with pLifeAct, F-actin is visualized using the fluorescence markers TagGFP2 or TagRFP**. Stable transfection of cell lines allows long-term actin staining for various applications.

Adenoviral vectors are available for difficult-to-transfect cells or when up to 100 % transgene expressing cells are needed. In this case, it is also possible to choose between the two promoters mentioned before.

In addition, different stable cell lines are available, which help facilitate daily lab life. You can immediately start performing experiments without the time-consuming and laborious generation of stable cell lines, while still assuring high-quality standards.
 

* Original LifeAct publication in Nature Methods:
Riedl J, et al. Lifeact – a versatile marker for the visualization of F-actin. Nature Methods 5, 605-607 (2008)

** Information about TagGFP2 and TagRFP at www.evrogen.com

LifeAct is a registered trademark of ibidi GmbH.

For license and product use information please refer to www.ibidi.com, Legal Notes.

Dr. Christiane Wiesner

“We use LifeAct-TagRFP and LifeAct-TagGFP2 plasmids in primary human macrophages. The LifeAct probes put us in the position to detect F-actin-rich adhesion structures, without the drawback of disturbing their dynamics. The signal is bright and clear without the background of nonintegrated G-actin. LifeAct gives us the opportunity to highlight F-actin-enriched, cytoskeletal organizations, without the disadvantages of fluorophore-tagged actin overexpression.”

Dr. Christiane Wiesner
University Medical Center
Hamburg-Eppendorf
Hamburg
Germany

www.linderlab.de

E-mail:
Password:
Forgot your password?
You do not have an account? Register now.

Your shopping cart is empty.

Please note: You need to be logged in to use the shopping cart.