• Fuse-It-P
  • Fuse-It-P

Fuse-It-P

A fusion reagent used to transfer water soluble proteins into the cytoplasm of living cells

  • 80-100 % efficiency independent of cell type, cell density, and developmental state
  • The lumen of fusogenic liposomes is used as a carrier for water soluble proteins
  • Fusion process completed within 1 - 20 minutes
  • Optimized for the transfer of low and intermediate protein amounts to prevent concentration-induced artifacts in cell behavior

in cooperation with

>> Order Your
Membrane Fusion
Free Sample

For pricing and online ordering, please log in.

Cat. No. Description Pcs./Box
60220 Fuse-It-P, lyophilized, for 100 µl solution, 3 mM, IR fluorescent 1
60221 Fuse-It-P, lyophilized, for 4 x 25 µl solution, 3 mM, IR fluorescent 1
60222 Fuse-It-P, lyophilized, for 400 µl solution, 3 mM, IR fluorescent 1
60223 Fuse-It-P, lyophilized, for 4 x 100 µl solution, 3 mM, IR fluorescent 1

Applications:

  • Transfer of labeled proteins or peptides into living cells for functional imaging, speckle analysis, FRAP, or single molecule analysis, etc.
  • Incorporation of antibodies or blocking antibodies into living cells
  • Blocking, induction, or replacement of protein induced/regulated signal cascades
  • Re-incorporation of proteins into mutant cells
 

Specifications:

Concentration 3 mM
Storage -20 °C
Shelf life 6 months
ExMax/EmMax 750/780 nm

NOTE: Transfer of heavily charged proteins can affect fusion efficiency.


Transferred proteins are instantly active inside the cells, and after membrane fusion, cells can immediately be used for further analysis. Since proteins are freed directly into the cytoplasm, no partial or complete lysosomal degradation can occur, as is typical for other endosomal uptake-depending proteofection methods. Depending on your experimental needs, the fusion process can be monitored by fluorescence microscopy.


 






Fuse-It-P vesicles were filled with LifeAct and fused with myofibroblasts. A bright staining of the
actin cytoskeleton can be observed after 5 minutes.

For details please watch the according videos:

Individual Staining

click here to enlarge

 

Overlay


click here to enlarge



The incorporation of small liposomal carriers into the plasma membrane of mammalian cells is the idea behind all of ibidi’s Fuse-It products. Liposomal carriers are able to attach and instantly fuse with plasma membranes in a physicochemical-driven manner. ibidi’s new Fuse-It reagents efficiently use this mechanism and fuse with mammalian cell surfaces immediately upon contact. Therefore, this novel technique makes the transfer of molecules independent of biological processes, such as endocytosis, pinocytosis, or specific receptor binding.
 



E-mail:
Password:
Forgot your password?
You do not have an account? Register now.

Your shopping cart is empty.

Please note: You need to be logged in to use the shopping cart.