• Fuse-It-L
  • Fuse-It-L

Fuse-It-L

A fusion reagent used to incorporate lipids or amphipathic molecules into the plasma membrane of living cells

  • Fusogenic liposomes used as a lipid carrier for the transfer into the cellular plasma membran
  • Superior fusion efficiency (up to 80-100%)
  • Fusion process completed within 1 - 10 minutes

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Cat. No. Description Pcs./Box
60210 Fuse-It-L, infrared fluorescent: lyophilized, for 100 µl solution (3 mM) 1
60211 Fuse-It-L, infrared fluorescent: lyophilized, for 4 x 25 µl solution (3 mM) 1
60212 Fuse-It-L, infrared fluorescent: lyophilized, for 400 µl solution (3 mM) 1
60213 Fuse-It-L, infrared fluorescent: lyophilized, for 4 x 100 µl solution (3 mM) 1

Applications:

  • Lipid imaging for monitoring turnover, traffic, and functional analysis in living cells
  • Lipid raft, lipid microdomain, and lipid diffusivity measurements
  • Blocking, induction, or replacement of lipid induced/regulated signal cascades
  • Re-incorporation of lipids into mutant cells affected in lipid biosynthesis


After membrane fusion, cells can immediately be used for further analysis. If necessary, more than one lipid of interest can be incorporated at once. Depending on your experimental needs, the fusion process can be monitored by fluorescence microscopy.

 

Specifications:

Form Lyophilized powder
Storage -20 °C
ExMax/EmMax 750/780 nm (infrared)


Bodipy FL C12-galactocerebroside and TopFluor cholesterol were incorporated
into the plasma membrane of CHO cells using Fuse-It-L (5 min incubation).



The incorporation of small liposomal carriers into the plasma membrane of mammalian cells is the idea behind all of ibidi’s Fuse-It products. Liposomal carriers are able to attach and instantly fuse with plasma membranes in a physicochemical-driven manner. ibidi’s new Fuse-It reagents efficiently use this mechanism and fuse with mammalian cell surfaces immediately upon contact. Therefore, this novel technique makes the transfer of molecules independent of biological processes, such as endocytosis, pinocytosis, or specific receptor binding.
 


Fuse-It membrane fusion

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