• Fuse-It-Beads
  • Fuse-It-Beads

Fuse-It-Beads

A fusion reagent used to transfer beads and nanoparticles into the cytoplasm of living cells

  • Efficient incorporation of beads and particles with diameters in the nm to µm range
  • Superior fusion efficiency (up to 80-100%)
  • Fusion process completed within 1 - 20 minutes

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Cat. No. Description Pcs./Box
60420 Fuse-It-Beads, infrared fluorescent: ready to use, 3 mM, 100 µl 1
60421 Fuse-It-Beads, infrared fluorescent: ready to use, 3 mM, 400 µl 1

Applications:

  • Magnetic µm-bead incorporation for magnetic tweezers experiments
  • Long-term live cell tracking
  • Cell purification assays using paramagnetic nm-beads
  • Incorporation of antibody-coupled particles for labeling, speckle microscopy, electron microscopy, etc.
  • Analysis of nanoparticle effects on cell behavior


After membrane fusion, the cells can immediately be used for further analysis. Multiple bead or single bead transfer is possible, depending on incubation time and bead concentration. Depending on your experimental needs, the fusion process can be monitored by fluorescence microscopy.

 

Specifications:

Form Solution
Concentration 3 mM
Storage -20 °C
ExMax/EmMax 750/780 nm (infrared)

Note: The transfer of positively charged beads can affect fusion efficiency.


CHO cells fused with Fuse-It-Beads for 2 minutes with M-270 magnetic Dynabeads.



The incorporation of small liposomal carriers into the plasma membrane of mammalian cells is the idea behind all of ibidi’s Fuse-It products. Liposomal carriers are able to attach and instantly fuse with plasma membranes in a physicochemical-driven manner. ibidi’s new Fuse-It reagents efficiently use this mechanism and fuse with mammalian cell surfaces immediately upon contact. Therefore, this novel technique makes the transfer of molecules independent of biological processes, such as endocytosis, pinocytosis, or specific receptor binding.
 


membrane fusion

Reinhard Windoffer, Institute for Molecular and Cellular Anatomy (MOCA), University Hospital RWTH Aachen, Germany

"The incorporation of magnetic beads into cells for use in our magnetic tweezers experiments was extremely difficult and stressful for our cells. With Fuse-It-Beads, we can now easily perform the incorporation within a few minutes, making our experiments much easier and more reliable.”

Dr. Reinhard Windoffer
Institute for Molecular and Cellular Anatomy (MOCA)
University
Hospital RWTH Aachen
Germany

www.ukaachen.de

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