• Fuse-It-B
  • Fuse-It-B

Fuse-It-B

A fusion reagent used to biotinylate the surfaces of living cells

  • Fusogenic liposomes are used as a carrier for the transfer of biotinylated lipids into the cellular plasma membrane
  • Superior fusion efficiency (up to 80-100%)
  • Fusion process completed within 1 - 20 minutes 

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Cat. No. Description Pcs./Box
60320 Fuse-It-B, green fluorescent: ready to use, 3 mM, 100 µl 1
60321 Fuse-It-B, green fluorescent: ready to use, 3 mM, 400 µl 1
60322 Fuse-It-B, infrared fluorescent: ready to use, 3 mM, 100 µl 1
60323 Fuse-It-B, infrared fluorescent: ready to use, 3 mM, 400 µl 1

Applications:

  • Cell surface functionalization and targeting using biotin-avidin coupling
  • Cell separation using avidin-coated magnetic beads
  • Attachment of living cells to distinct precoated surfaces
 

Specifications:

Form Solution
Concentration 3 mM
Storage -20 °C
ExMax/EmMax 484/501 nm (green)
750/780 nm (infrared)


After membrane fusion, the cells can immediately be used for further analysis. Biotin can be visualized by anti-biotin antibodies or bound to avidin, neutravidin, and streptavidin, respectively. Depending on your experimental needs, the fusion process can be monitored by fluorescence microscopy.


 






CHO cells fused with Fuse-It-B (red) and subsequent binding of avidin-alexa488 (green)



The incorporation of small liposomal carriers into the plasma membrane of mammalian cells is the idea behind all of ibidi’s Fuse-It products. Liposomal carriers are able to attach and instantly fuse with plasma membranes in a physicochemical-driven manner. ibidi’s new Fuse-It reagents efficiently use this mechanism and fuse with mammalian cell surfaces immediately upon contact. Therefore, this novel technique makes the transfer of molecules independent of biological processes, such as endocytosis, pinocytosis, or specific receptor binding.
 


Fuse-It membrane fusion

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