Collagen Type I

A high quality collagen solution used for creating 3D collagen gels (e.g., in chemotaxis assays)

Provides in vivo–like ECM (extracellular matrix) structures
High concentrated collagen solution – usable for a variety of gel concentrations
Fast polymerizing for maximal cell distribution in gel
High purity for well-defined 3D collagen gels

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Cat. No.	Description	Pcs./Box
50201	Collagen Type I, rat tail, non-pepsinized, 5 mg/ml, 1 x 5 ml	1
50202	Collagen Type I, rat tail, non-pepsinized, 5 mg/ml, 4 x 5 ml	1
50203	Collagen Type I, rat tail, non-pepsinized, 5 mg/ml, 1 x 100 ml	1

Applications:

Chemotaxis assays in firm 3D collagen gels
Leukocyte and tumor cell migration
3D cell microscopy
Culturing cells on thin or thick collagen layers
Coating of cell cultureware to promote cell attachment
Differentiation studies – 2D versus 3D
Highly suited for live cell imaging and fluorescence microscopy

Technical Features:

High-quality purified protein from rat tendons
Ideal for use in combination with µ-Slide Chemotaxis^3D
Ready-to-use
Highly standardized manufacturing process
Stringent quality standards
Simple and easy protocol and handling
Storage at -20°C for well-defined quality and reproducibility

Specifications:

Source	Rat tail tendon
Appearance	Optically clear viscous liquid
Extraction	Acid, non-pepsinized
Purity	> 90 % by SDS PAGE
Sterility	Sterile, for cell culture
Contaminants	Negative for DNA, bacteria, fungi, and mycoplasma
Growth factors	None
Formulation	Supplied in 2 mM acetic acid (0.1%)
pH	pH 3.8
Functional control	3D gelling and 2D coating test in cell culture
Storage	-20°C

Application Examples

Chemotaxis of HT-1080 cells,
which are migrating towards FCS,
using low resolution microscopy
for a maximum of migration data

High resolution microscopy during chemotaxis and cell polarization, using the HT-1080 LifeAct cell line

Cell spheroid (or micro tumor) on top of, or embedded in, a 3D matrix for long-term cultivation. The upper well provides nutrients by means of diffusion.