ibiClone^TM Adeno Adenovirus Cloning Kit

A kit for recombination-based, adenoviral vector generation in E.coli, using BAC recombination technology

Extremely fast vector cloning and production in 2 weeks
Reliable results due to the sophisticated BAC selection system
Highest cloning efficiency available using BAC technology (100% stable, positive clones)
Simple and ready-to-use kit

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Cat. No.	Description	Pcs./Box
60661	ibiClone Adeno Adenovirus Cloning Kit: pO6A5 shuttle vector for gene overexpression (CMV promoter), electrocompetent BA5 FRT cells, 10 reactions	1
60662	ibiClone Adeno Adenovirus Cloning Kit: pO6A5 shuttle vector for silencing (U6 promoter), electrocompetent BA5 FRT cells, 10 reactions	1
60663	Consumables ibiClone Adeno Adenovirus Cloning Kit: electrocompetent BA5 FRT cells, 10 reactions	1

Applications:

Gene (over)expression in mammalian cells
Gene knockdown in mammalian cells

NOTE:
Please note that you will be working with samples containing an infectious virus. Follow the recommended NIH guidelines for all materials containing BSL–2 organisms.

Specifications:

Kit Components	Shuttle vector pO6A5-CMV E.coli BA5-FRT electrocompetent cells SOC medium
Amount	10 reactions
Storage	Bacteria: -80 °C Plasmid: -20 °C SOC medium: Room temperature

Technical Features:

A patent-pending adenovirus vector technology
100% stable clones created through BAC technology
100% positive clones created through BAC technology

The ibiClone™ Adeno Cloning System is a novel, innovative technology that allows for the fast and convenient generation of recombinant adenoviruses, in as little as two weeks. The system is basically composed of just two major elements:

Component 1: A small shuttle vector (pO6A5), in which the transgene is cloned.

Component 2: Electrocompetent BA5-FRT E. coli cells with a BAC vector that confers resistance to chloramphenicol. Contained on this BAC vector is an Ad5 genome that is devoid of the left ITR, the packaging signal, the E1 sequences, and 720 bp of the E3 region.

Following the transformation of the shuttle vector into the electrocompetent BA5-FRT E.coli cells, an Flp recombinase-mediated recombination occurs between the shuttle and the BAC vector.

Due to a highly sophisticated selection system, only cells containing a recombined BAC vector can grow and form bacterial colonies. As a result, almost 100% of the screened colonies contain BAC vectors with the correctly recombined transgene. Because of this factor, the ibiClone™ Adeno Cloning System requires very limited ‘hands on’ time, from the shuttle vector transformation to the isolation of virus particles. Virus yields and quality are absolutely comparable to those obtained with established adenoviral cloning systems.