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ibidi FAQs

Find answers to frequently asked questions here

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Is the ibidi Heating & Incubation System compatible with every type of microscope?

The ibidi Heating & Incubation System 1, universal fit, is compatible with any inverted microscope that has a stage or a frame for multi-well plates, such as 96-well plates. The outer dimensions of this ibidi Heating System and a 96-well plate are identical.

Additionally, ibidi has developed a customized heating system for the Nikon TI-S-ER Motorized Stage which holds multi-well plates. Thus, the system permits the user to control the temperature of samples held in multi-well plates.

Is it possible to incubate multi-well plates?

ibidi has developed a customized heating system for the Nikon TI-S-ER Motorized Stage which holds multi-well plates. Thus, the system permits the user to control the temperature of samples held in multi-well plates.

If you would like further information on adapting your current heating system, please contact us.

Does the heated lid of the ibidi Heating & Incubation System affect resolution?

No. There is no reported effect on the optical pathway, because the heated lid in the ibidi Heating & Incubation System is made of glass. So, the resolution remains unchanged. In theory, there is a small percentage of intensity loss during light transmission, but this is not a problem for transmitted light. When working with fluorescence microscopy, the lid is not inside the optical pathway.

Can you recommend a good stage warmer that would not lessen the quality of imaging?

We developed the ibidi Heating & Incubation Systems exactly for this purpose. They work like small stage top incubators, which are made for live cell imaging.

Can ibidi Heating Systems be combined with gas incubation?

The ibidi Heating & Incubation Systems are designed for use in combination with ibidi gas incubation. For this purpose, there are two openings in the heated lid, one for the gas inlet and the other for the humidity sensor. This allows not only temperature, but also the CO2 concentration and the humidity to be controlled. All ibidi temperature chambers are upgradeable for gas incubation.

Is oil-immersion microscopy possible with the ibidi Heating System?

Yes, the ibidi Heating & Incubation System does not add any obstacle between the sample and the objective lens. This is due to the opening.

Is the stability of the focal plane, when affected by temperature variations, sufficient for high resolution and confocal microscopy?

Yes. To precisely stabilize the focal plane, you need to maintain a temperature with an accuracy of 0.1°C minimum. The ibidi Temperature Controller has the capability to maintain temperature stability of ± 0.05 °C.

Can I be sure that the temperature indicated on the controller is the same as in the immediate vicinity of the cells?

Not 100%. Technically, it is not possible for us to place a sensor close to the cell position. Therefore, the measured temperature is the temperature of the metal base plate, or the heated lid respectively. To make sure that your cells are at the desired temperature, we recommend calibrating the system each time you change your setup. Temperature changes can occur when you exchange the type of sample holder (e.g., from µ-Dish to µ-Slide 8 Well), or when switching microscopes. To calibrate the system, you can use the thermometer that was provided for the sensor and which is placed in the sample well.

Which immersion oils are compatible with ibidi products?



Ordering Number


ibidi Immersion Oil

(ibidi) 50101


Immersol 518 F

(Zeiss) 444960


ImmersolW 2010

(Zeiss) 444969


Immersion liquid

(Leica) 11513859

Which hardware autofocus is compatible with the ibidi µ-Dishes and µ-Slides?

Due to the standard refractive index of nD=1.52, the ibidi Standard Bottom is compatible with all hardware focus systems, such as the Nikon Perfect Focus System (PFS).
Note: Some hardware focus setups measure the air-medium interface, which is absent in channel µ-Slides. In this case, the autofocus system may need to be adjusted to detect the correct surface. Please contact us for detailed troubleshooting.