Is it possible to use Wimasis Image Analysis for analyzing cell migrations in vitro/vivo? Should it be only on the skin, or can it be deeper in the tissues?

Wimasis, as a service provider, develops solutions for both areas, in vitro and in vivo (99% tissue outside the organism). For migration in vitro, there are two main tools available. The linear migration, in bulk, is available with the Wound Healing Image Analysis tool, WimScratch. The software tool for tracking single cell migration in 2D is available with the Chemotaxis Image Analysis tool, WimTaxis.

Regarding the second question, “on the skin or deeper in the tissue”, the answer depends on your experimental setup. Wimasis has worked with many variations so far, and can only recommend that you implement a feasibility study when planning and conducting the experiment.

Is the Wimtaxis software still only semi-automatic? How long does the process usually take?

The analyses for wound healing (WimScratch) and tube formation (WimTube) assays are already completely automated. However, the chemotaxis tool, WimTaxis, is currently only semi-automatic, which can mean a longer waiting time for your results. Still, Wimasis is working hard to provide analysis within a few days.

Does Wimasis only work with phase contrast images, or with Differential Interference Contrast (DIC) and fluorescence images, as well?

The Wimasis standard WimScratch and WimTube solutions are optimized for phase contrast images. However, as Wimasis has a lot of experience with DIC and fluorescence, they are able to adapt the algorithm within a day. Please contact them for more information.

Which magnification does Wimasis recommend?

The standard solutions, WimScratch and WimTube, are optimized for low (~5x), medium (~10x), and high (~20x) magnification.

Will Wimasis save all of the information and results from my experiment, or will it be deleted after the analysis?

Wimasis stores the results for 90 days, on an anonymous and non-sorted database, to improve the algorithms. After 90 days, all data will be deleted.

Is the Wimasis solution only associated with ibidi products?

No. Wimasis’ algorithms are developed with many research groups worldwide, and not all of them use ibidi products in their experiments. ibidi’s goal is to help you achieve better image quality for your experiments. To this end, Wimasis will independently analyze the image results from your cell culture experiments. 

To have some of your own cell culture experiments analyzed, just upload the images to the individual image analysis platforms for wound healing, tube formation, or chemotaxis

Is Wimasis planning to analyze other assays?

At the moment, Wimasis is planning a cooperative program on the study of autophagy and apoptosis. We suggest that you check out the Wimasis website  from time to time for new information or subscribe to the Wimasis newsletter in the News and Events section.