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Transfection, Transduction, and Membrane Fusion

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Application Notes

Gene Transfection (AN 07)
Example showing how protocols for gene transfection can easily be adapted to the work in cell culture channels

Adenoviral Transduction of Human Cells (AN 28)
Detailed protocol for handling recombinant adenoviruses, plus designing an approach for transducing human cells

Lentiviral Transduction of Human Cells (AN 41)
Detailed protocol for handling recombinant lentiviruses, plus designing an approach for transducing human cells

AN 52: Transfer of R-Phycoerythrin into CHO-K1 cells using Fuse-It-P (AN 52)
Detailed instructions for the transfer of the protein R-Phycoerythrin into CHO-K1 cells using Fuse-It-P in the µ-Dish 35 mm, high

Transfer of mRNA into L929 Cells using Fuse-It-mRNA (AN 43)
Detailed protocol for handling recombinant lentiviruses, plus designing an approach for transducing human cells

Transfer of mRNA into iPSC-derived Cardiomyocytes Using Fuse-It-mRNA (AN 29)
Detailed instructions for the optimization of the Fuse-It-mRNA protocol for iPSC-derived Cardiomyocytes in the µ-Slide VI 0.4