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Can Immunofluorescence be Fun?

Immunofluorescence techniques are widely used in cell biology and tissue samples. ibidi provides solutions to facilitate these assays without compromising high resolution imaging results. Main advantages are:

  1. Fast and simple preparation
    All-in-one chambers simplify your immunofluorescence protocol
  2. Homogeneous cell distribution
    Cell densities are independent of handling during cell seeding
  3. Cost-effective experiments
    Only a small number of cells and low volumes of reagents are needed
  4. High resolution imaging
    Can be used for wide-field fluorescence, confocal imaging, FRAP – FRET – FLIM, and undisturbed phase contrast imaging

Every fluorescence protocol consists of four major steps: cultivation, fixation, staining, and sub­sequent imaging of the cells. Unlike most plastic materials used for cell culture, the ibidi μ-Slides and µ-Dishes are compatible with alcohols, acetone, acids, and para-formaldehyde. Therefore all steps can be performed directly in the slides or dishes.


Eliminate the Need for Tweezers in IF Protocols

The protocols below show that the ibidi method for performing immunofluorescence assays is superior to the traditional coverslip method.

The main advantages of the ibidi method are faster preparation and easier paral­lelization of assays. In most cases, channel slides are recommended for use in immunofluorescence assays (e.g., µ-Slides VI 0.4 or µ-Slide I 0.4 Luer). When working with these types of slides, never again tweezers will be needed in IF protocols. If needing to store samples for several years, the removable Immunofluorescence Chambers are perfectly suited for this purpose. Their re­movable chambers can be sealed for long-term storage by mounting a coverslip using the old-fashioned nail polish technique.

Protocol with ibidi µ-Slides

(New method using all-in-one chambers)

Protocol with Cells on Coverslips

(Traditional method with nail polish mounting)

  • Sterilize coverslips and slides
  • Coat coverslips
  • Place sterile coverslips into 6-well plate
  • Seed cells in large volume
  • Peel off the coverslip
  • Wash
  • Fix – wash – permeabilize – wash - block
  • Wash
  • Stain cells
  • Wash
  • Mount cells with mounting medium
  • Mount coverslip with nail polish
  • Sterilize coverslips and slides
  • Coat coverslips
  • Place sterile coverslips into 6-well plate
  • Seed cells in large volume
  • Peel off the coverslip
  • Wash
  • Fix – wash – permeabilize – wash – block
  • Wash
  • Stain cells
  • Wash
  • Mount cells with mounting medium
  • Mount coverslip with nail polish