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Application Notes

Chemotaxis 2D and 3D (AN 17)
General protocol for 2D and 3D gel assays with µ-Slide Chemotaxis

3D Chemotaxis Protocol for Non-Adherent Cells in a Gel Matrix (AN 23)
Application Note providing a specific example protocol for chemotaxis of dendritic cells in a collagen gel

Chemotaxis Assay with Cells in the Reservoirs (AN 35)
A detailed protocol for using adherent cells as chemoattractant-producers in the large reservoirs of the µ-Slide Chemotaxis.

Chemotaxis of HT-1080 Cells in 2D and 3D (AN 24)
Detailed protocol of experimental parameters and example data of HT-1080 cells migrating in 2D and in 3D in collagen I gels

Chemotaxis of HUVEC Cells in 2D and 3D (AN 34)
Detailed protocol of experimental parameters and example data of HUVEC cells migrating in 2D and in 3D in collagen I gels.

Image Shift Correction in Microscopic Time Lapse Sequences (AN 37)
Instructions for correcting an externally generated shift of the sample in time lapse images (e.g. in chemotaxis experiments)