Cell Culture Under Flow
- Which specific applications use the µ-Slide VI 0.1?
- Can the µ-Slide III 3in1 be controlled by using the ibidi Pump System?
- Is the flow pattern in µ-Slide y-shaped turbulent?
- Can I use any slides, other than the ibidi µ-Slides, with the ibidi Pump System?
- How much shear stress is suitable for rolling and adhesion assays?
- Can rolling and adhesion assays be performed using the ibidi Pump System?
- Why are my cells not elongated under flow conditions?
- What is the difference between the shear stress in the branched regions and the single channel of the µ-Slide y-shaped?
- How long should the shear stress be applied to the cell layer before doing the analysis?
- How much shear stress should be applied to endothelial cells?
- How can the number of cells in the flow assay be increased?
- How long should the cells attach before starting the flow?
- How can the silica beads be regenerated?
- How can the components of the ibidi Pump System be cleaned?
- How often can the Perfusion Sets be reused?
- Which parts of the ibidi Pump System have to be maintained and can be exchanged?
- How is the sterility within the perfusion system maintained?
- How is ensured that the cells are supplied with incubated air?
- Which components of the ibidi Pump System have to be placed inside the incubator during operation?
- Which upgrades are available for the ibidi Pump System?
- Which flow types are possible with the ibidi Pump System?
- Is the ibidi Pump System a stand-alone system?
- Can the ibidi Pump System be used to measure flow and wave profiles?
- How can shear stress be calculated for suspension cells in channel slides?