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What Type of Assay Are You Planning?

Flow Conditioning of an Endothelial Cell Layer

Application: Endothelial cells under shear stress conditions

  • Investigating the influence of shear stress
    on endothelial cells
  • Preparing cells while mimicking in vivo perfusion conditions
  • Investigating platelet or leukcyte adhesion to endothelial cells cultured under in vivo-like conditions
  • Creating biofilms of microorganisms
  • Staining antibodies
Flow Characteristics: Continuous laminar, non-uniform, oscillating (needed for turbulence simulation), pulsatile
Duration: Hours, up to several weeks
Experimental Environment: Incubation conditions
Recommended Pumps: ibidi Pump System
Recommended μ-Slides: μ-Slide VI 0.4, μ-Slide I Luer Family

Rolling and Adhesion Assays

Application: Rolling and adhesion of suspended cells to protein surfaces

  • Blood cells (e.g., platelets, leukocytes) on adhesion proteins
  • Blood cells on flow conditioned cell monolayers
Flow Characteristics: Continuous laminar
Duration: Minutes to hours
Experimental Environment: Room temperature or incubation conditions
Recommended Pumps: ibidi Pump System, Syringe pumps, Peristaltic pump
Recommended μ-Slides: μ-Slide VI 0.4, μ-Slide I Luer Family, μ-Slide y-shaped

Stop Flow Experiments

Application: Defined liquid exchange

  • Ca2+-imaging
  • Defined medium exchange for optimal feeding
  • Online stimulation of adherent cells with drugs
  • Live staining
Flow Characteristics: Laminar flow with short periods of flow in between non-flow phases
Duration: Minutes to hours
Experimental Environment: Room temperature or incubation conditions
Recommended Pumps: Manual liquid delivery, Syringe pumps,Peristaltic pump
Recommended μ-Slides: μ-Slide VI 0.4, μ-Slide I Luer Family

3D Cell Culture: Interstitial Flow

Application: 3D cultures of cells and tissue in gel matrices:

  • Hepatocytes
  • Fibroblasts
  • Muscle cells
  • Kidney cells
  • Stem cells
Flow Characteristics: Continuous interstitial or stop flow interstitial
Duration: Hours, up to several weeks
Experimental Environment: Incubation conditions
Recommended Pumps: ibidi Pump System
Recommended μ-Slides: μ-Slide VI 0.4, μ-Slide I Luer Family

ECIS Flow Assays

The ECIS technology permits the measurement of even small morphological changes in electrical signals in an adherent cell layer. It works without disturbance to the cells, and without requiring any staining. The measurements are automated and are therefore highly reproducible with minimum effort.

The ECIS Flow Array enables researchers to investigate cells under both perfusion and defined shear stress conditions.

More Information:

Find detailed information in Application Note 11: Shear Stress/Shear Rates and Flow Rates in ibidi’s Channel Slides

Watch our movie Long-Term Cell Culture Under Perfusion (MV 21) to get more information on applications and handling.

For additional information, please also listen to ibidi’s recorded webinars "Cell Cultivation under Flow".

Register for a 2 day Laboratory Course at ibidi Munich / Germany: Cell Cultivation under Perfusion and Live Cell Imaging.

Contact ibidi for a free demo of the ibidi Pump System.