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Cell Attachment

Impedance Measurements

The interaction between surfaces and cells is a main component of cell behavior in vivo and in vitro and therefore an area of major interest in cell biology studies. ECIS allows researchers to study and quantify the interaction of cultured cells with extracellular matrix proteins, and other macromolecules by taking continuous, real-time impedance measurements.


Used electrode arrays for cell attachment & spreading:

8 well format:

  • 8W10E
  • 8W10E+

96 well format:

  • 96W10E

Optical Measurements

Optical measurements of cell attachment rates are taken using ibidi’s μ-Slide VI 0.4 and μ-Slide Angiogenesis.

cell_attachment2.jpg


Toxicological Screening

Impedance Measurements

Studies measuring well-defined dose- and time-related toxic effects of chemicals often employ small numbers of cells during testing, and ECIS has been successfully used in such studies. ECIS effectively describes the relationship between dose and its effects on the exposed organism, e.g., comparisons of LD50 (Lethal Dose, 50 %) values, toxicological effects from heavy metals, and the effects of viral infections on cell populations.

Cells challenged with decreasing concentrations of a virus

The following electrode arrays are ideally suited for toxicological screenings:

8 well format:

  • 8W10E
  • 8W10E+

96 well format:

  • 96W10E+

Optical Measurements

Optical measurements of toxicology are possible with all ibidi μ-Slides and μ-Dishes.


Signal Transduction

Impedance Measurements

ECIS allows monitoring the signal transduction pathways activated by G protein coupled receptors (GPCR). This assay is based upon the widely accepted conjecture that GPCR activation, regardless of the second messenger, results in alterations of the cell's cytoskeletal elements. This culminates in morphological changes, and this is precisely the type of event detected in real-time and with great sensitivity by the ECIS System.

Experimental Example

Changes in barrier function in human dermal microvascular endothelial cells (HDMECs) measured as resistance at 2000 Hz due to addition of:

serum free medium (black line),
2.5 µM Thapsigargin (red line),
12.5 nM Histamine (blue line),
and 12.5 nM Histamine after incubation in 2.5 uM Thapsigargin (dotted blue line).

The following electrode arrays are ideally suited for signal transduction studies:

8 well format:

  • 8W10E
  • 8W10E+

96 well format:

  • 96W10E+