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µ-Slide Chemotaxis

The μ-Slide Chemotaxis was developed to investigate the chemotactical behavior of fast or slow migrating, non-adherent or adherent cells on 2D surfaces or in 3D gel matrices. It is possible to observe the migration in linear and stable concentration profiles for over 48 hours. As gradients are rapidly established, fast responses (occurring in less than 30 minutes) can also be measured.

The µ-Slide Chemotaxis is for use with all adherent cells that will be observed in 2D or in in vivo-like 3D gel conditions, such as endothelial cells, fibroblasts, cancer cells, and others and non-adherent cells, such as T-cells, dendritic cells, neutrophils, monocytes, granulocytes, lymphocytes, and others.

Principle:

A narrow observation area connects two large reservoirs. The cells that are embedded in a gel inside the observation area become super-imposed by linear and time-stable gradients.

Chemotaxis is observed using time-lapse microscopy.

Observation area of the µ-Slide Chemotaxis recorded with a 4 x objective for a maximum of migration data.

Dendritic cell (mouse) with LifeAct-labeled F-actin that is migrating towards CCL 19 inside a Collagen I gel matrix. Spinning disc confocal microscopy (objective lens 63x).

Chemotaxis of HT-1080 cells, on a Collagen IV coated 2D surface (left) and a Collagen I 3D gel (right) migrating towards FCS (objective lens 10x).

Gradient Stability

The ibidi μ-Slide Chemotaxis is designed to provide a quick gradient with excellent long-term gradient stability for over 48 hours. The chamber design is especially made for working with fast (e.g., leukocyte) or slow migrating (e.g., cancer) cells. When working with aqueous gels like Collagen I or Matrigel, the gradient establishment is not hindered by the gel.

Gradient measurement across the observation area in the µ-Slide Chemotaxis.

More information:

For practical details please watch the ibidi movies

In various Application Notes you will find detailed information on how to perform your chemotaxis experiments.