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Data Acquisition and Analysis

Data Acquisition

Microscopy is used to evaluate the tube formation process. Depending on your focus of interest, this can either be done by using video microscopy (a continuous movie), or by observing images at distinct time points (e.g., after 6 hours and 12 hours). The ibidi Heating & Incubation Systems enable live cell imaging with incubator conditions directly on the microscope.

One well of the µ-Slide Angiogenesis, with HUVEC cells on Matrigel after 12 hours of incubation.

Characteristic pattern of human endothelial cells (HUVEC), stained with Calcein.

Data Analysis

Tube Formation Image Analysis is a crucial step in angiogenesis assays. The Tube Formation ACAS Image Analysis is an analysis solution to quantitatively evaluate the generation of cellular networks.
First, use the µ-Slide Angiogenesis or the µ-Plate Angiogenesis 96 well to perform your tube formation experiments and acquire microscopy images. After acquiring the microscopy images, simply upload your data into your ACAS account and start the analysis job. Within minutes, you will receive a detailed report for download and further evaluation.

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Reports with Analyzed Data Contain:

  • Loop count
  • Average loop area
  • Average tube length (per loop)
  • Total tube length
  • Branch count

 

More information:

ibidi’s Application Note Data Analysis of Tube Formation Assays (AN 27) shows you how to optimize the setup of angiogenesis assays, and how to analyze and interpret the data.

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Inhibition of tube formation by a compound in a dose-effect relation