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APPLICATION NOTE

ibidi’s Application Note Wound Healing Assay - Data Analysis (AN 30) shows you how to optimize the setup of wound healing assays, and how to analyze and interprete the data.

ibidi FAQs

Find answers to frequently asked questions on wound healing and migration assays.

Impedance Measurements

The ECIS Wound Healing Assay replaces the traditional scratch assay. Instead of mechanically disrupting the cell layer with a pipette tip, and then following the migration of cells with a microscope, the ECIS System uses electric signals to both wound and then monitor the healing process.

The advantages of an impedance-based wound healing assay include:

  • up to 96 simultaneous wound healing experiments
  • the direct derivation of time constants and wound healing velocity
  • a cost-efficient approach to screening applications, from low to high throughput

Experimental Example: Healing Function of Serum

Electrical wounding is performed on a small population of cells that are in contact with a 250 μm electrode, thus creating a well-defined wound.

After the wounding, the healthy cells around the electrode immediately migrate and replace the dead cells on the electrode. Depending on the fetal calf serum (FCS) concentration, the original plateau is once again reached after 10, 13, or 20 hours.