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Order a FREE SAMPLE to test ibidi’s µ-Slides, µ-Dishes, and µ-Plates with your experiments.

APPLICATION NOTE

ibidi’s Application Note Wound Healing Assay - Data Analysis (AN 30) shows you how to optimize the setup of wound healing assays, and how to analyze and interprete the data.

ibidi FAQs

Find answers to frequently asked questions on wound healing and migration assays.

Data Acquisition and Analysis

Data Acquisition

Microscopy is used to evaluate the wound healing process. Depending on your focus of interest, this can either be done by using video microscopy, or by observing images at distinct time points (e.g., after 6 and 12 hours). The ibidi Heating & Incubation Systems enable live cell imaging with incubator conditions directly on the microscope.


Data Analysis

ibidi’s Wound Healing Image Analysis solution evaluates 2D cell migration and provides results in only 4 steps.

The web-based automated WimScratch software generates results from your collected data within minutes. Simply upload your data to the image analysis platform and instantly receive results via E-mail.

Analysis data contain:

  • Cell-covered area
  • Speed of closure (available ≥ 5 images)
  • Acceleration characteristics (available ≥ 5 images)
  • Overview chart
  • Center piece approximation (available ≥ 10 images)