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APPLICATION NOTE

The Application Note Tube Formation in µ-Plate Angiogenesis 96 well provides practical advice on how to run tube formation assays using the µ-Plate Angiogenesis 96 well.

ibidi MOVIE



For practical details please watch our movie Performing an Angiogenesis Assay (MV 15).

Tube Formation Assays in the
µ-Plate Angiogenesis 96 well

By utilizing ibidi’s μ-Plate Angiogenesis 96 well the anti-angiogenic potential of substances can be tested with functional tube formation assays. With this fully SBS (Society for Biomolecular Screening) compliant and robotics compatible screening plate, compounds can be tested for their influence on structural functionality, using an in vitro assay. The most significant example of such an assay, i.e., the functional tube formation assay, using endothelial cells on Matrigel™*, is described below.

The μ-Plate Angiogenesis 96 well uses the proven “well-in-a-well” feature to avoid meniscus formation in individual wells.

Other advantages include:

  • 90 % less Matrigel is needed, compared to using standard 96-well plates
  • Allows for efficient imaging as all cells are in one focal plane

Furthermore, only four steps are needed to go from sample preparation to image analysis:

Typical Screening Data Obtained with the μ-Plate Angiogenesis 96 well

The tube length was measured after 6 hours. The graph shows that different inhibitors act on the mechanism of tube formation in varying degrees and inhibit the formation of characteristic patterns.

*Matrigel is a registered trade mark of Becton Dickinson.