ibidi TIP

For more than 500 scientific publications using the ECIS technology please visit www.biophysics.com.

Signal Transduction

Impedance Measurements

ECIS allows monitoring the signal transduction pathways activated by G protein coupled receptors (GPCR). This assay is based upon the widely accepted conjecture that GPCR activation, regardless of the second messenger, results in alterations of the cell's cytoskeletal elements. This culminates in morphological changes, and this is precisely the type of event detected in real-time and with great sensitivity by the ECIS System.

Changes in barrier function in human dermal microvascular endothelial cells (HDMECs) measured as resistance at 2000 Hz due to addition of:
serum free medium (black line),
2.5 µM Thapsigargin (red line),
12.5 nM Histamine (blue line),
and 12.5 nM Histamine after incubation in 2.5 uM Thapsigargin (dotted blue line).

The following electrode arrays are ideally suited for signal transduction studies:

8 well format:

  • 8W10E
  • 8W10E+

96 well format:

  • 96W10E+