FREE SAMPLES

Order a FREE SAMPLE to test ibidi’s µ-Slides, µ-Dishes, and µ-Plates with your experiments.

ibidi CALENDAR

Submit your immunofluorescence images to ibidi and place your most successful experiments on your lab’s wall.

Removable Chambers for IF

ibidi also provides the easiest and most convenient solution for removable culture wells. These products contain a silicone gasket with chambers for individual cell culture and incubation conditions. The ibidi micro- Insert 4 well allows the user to employ extremely small volumes of medium and cell numbers for cell cultivation. The 12 well Chamber, removable is perfect for long-term storage of samples prepared using the classical “glass slide with mounted coverslip” technique.

Please refer to the overview table for technical details for these products.

Different Protocols Possible when Using the 12 well Chamber, removable


The technique employed to use the 12 well Chamber, removable is similar to the standard method. The cells are cultured in the wells of the 12 well Chamber’s silicone gasket using a culture volume of 250 μl. For immuno-fluorescence staining, the user can then employ any one of the protocols shown in the diagram on the right.

Advantages of Removable Chambers

  • The micro-Insert 4 well requires extremely small volumes of reagents and small numbers of cells for cultivation and staining
  • The 12 well Chamber, removable, is perfect for long-term storage of samples
  • The 12 well Chamber, removable, allows the user to perfom up to 12 parallel tests on one slide
  • Sterile and ready to use

Limitations of Removable Chambers

  • The 12 well Chamber, removable, requires large volumes of reagents and high numbers of cells for cultivation and staining