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Removable Chambers for IF

ibidi also provides the easiest and most convenient solution for removable culture wells. These products contain a silicone gasket with chambers for individual cell culture and incubation conditions. The ibidi micro- Insert 4 Well allows the user to employ extremely small volumes of medium and cell numbers for cell cultivation. The Immunofluorescence Chambers Family, e.g., the 12 Well Chamber, removable, is perfect for long-term storage of samples prepared using the classical “glass slide with mounted coverslip” technique.

Please refer to the overview table for technical details for these products.

Different Protocols Possible when Using the Immunofluorescence Chambers

The technique employed to use the Immunofluorescence Chambers is similar to the standard method. The cells are cultured in the wells of the 3 Well, 8 Well, or 12 Well Chamber’s silicone gasket. For immunofluorescence staining, the user can then employ any one of the protocols shown in the diagram below.

Advantages of Removable Chambers

  • The micro-Insert 4 Well requires extremely small volumes of reagents and small numbers of cells for cultivation and staining
  • The Immunofluorescence Chambers Family is perfect for long-term storage of samples
  • The Immunofluorescence Chambers Family allows the user to perfom up to 12 parallel tests on one slide
  • The Immunofluorescence Chambers Family combines a large growth area with a small staining volume (especially 3 Well Chamber, removable)
  • Sterile and ready to use

Limitations of Removable Chambers

  • The Immunofluorescence Chambers Family requires high numbers of cells for cultivation and staining.