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Removable Chambers for IF
ibidi also provides the easiest and most convenient solution for removable culture wells. These products contain a silicone gasket with chambers for individual cell culture and incubation conditions. The ibidi micro- Insert 4 well allows the user to employ extremely small volumes of medium and cell numbers for cell cultivation. The removable μ-Chamber 12 well is perfect for long-term storage of samples prepared using the classical “glass slide with mounted coverslip” technique.
Please refer to the overview table for technical details for these products.
Different Protocols Possible when Using the µ-Chamber 12 well
The technique employed to use the µ-Chamber 12 well is similar to the standard method. The cells are cultured in the wells of the μ-Chamber 12 well’s silicone gasket using a culture volume of 250 μl. For immunofluorescence staining, the user can then employ any one of the protocols shown in the diagram on the right.
Advantages of Removable Chambers
- The micro-Insert 4 well requires extremely small volumes of reagents and small numbers of cells for cultivation and staining
- The μ-Chamber 12 well is perfect for long-term storage of samples
- The μ-Chamber 12 well allows the user to perfom up to 12 parallel tests on one slide
- Sterile and ready to use
Limitations of Removable Chambers
- The μ-Chamber 12 well requires large volumes of reagents and high numbers of cells for cultivation and staining