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APPLICATION NOTE

Find detailed immunofluorescence staining protocols in ibidi’s Application Notes:

AN 02: µ-Slide I (PDF)
AN 09: µ-Slide VI 0.4 (PDF)
AN 15: µ-Slide y-shaped (PDF)
AN 16: µ-Slide 8 well (PDF)

ibidi CALENDAR

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Eliminate the Need for Tweezers in IF Protocols

The protocols below show that the ibidi method for performing immunofluorescence assays is superior to the traditional coverslip method.

The main advantages of the ibidi method are faster preparation and easier paral­lelization of assays. In most cases, channel slides are recommended for use in immunofluorescence assays (e.g., µ-Slides VI 0.4 or µ-Slide I 0.4 Luer). When working with these types of slides, never again tweezers will be needed in IF protocols. If needing to store samples for several years, the µ-Chamber 12 well is perfectly suited for this purpose. Its re­movable chambers can be sealed for long-term storage by mounting a coverslip over the chambers using the old-fashioned nail polish technique.

Protocol with ibidi µ-Slides

(New method using
all-in-one chambers)

 

Protocol with Cells on Coverslips

(Traditional method
with nail polish mounting)


  • Sterilize coverslips and slides
  • Coat coverslips
  • Place sterile coverslips into 6-well plate
  • Seed cells in large volume
  • Peel off the coverslip
  • Wash
  • Fix – wash – permeabilize – wash - block
  • Wash
  • Stain cells
  • Wash
  • Mount cells with mounting medium
  • Mount coverslip with nail polish

 

  • Sterilize coverslips and slides
  • Coat coverslips
  • Place sterile coverslips into 6-well plate
  • Seed cells in large volume
  • Peel off the coverslip
  • Wash
  • Fix – wash – permeabilize – wash – block
  • Wash
  • Stain cells
  • Wash
  • Mount cells with mounting medium
  • Mount coverslip with nail polish