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APPLICATION NOTE

In our Application Note Chemotaxis 3D (AN 17) you will find a general protocol for 3D gel assays using the µ-Slide Chemotaxis 3D.

ibidi FAQs

Find answers to frequently asked questions on chemotaxis assays.

PRACTICAL COURSE

Register for a 2 day Laboratory Course at ibidi Munich / Germany: Chemotaxis Assays and Video Microscopy.

µ-Slide Chemotaxis 3D

The μ-Slide Chemotaxis 3D was developed to investigate the chemotactical behavior of fast or slow migrating, non-adherent cells in gel matrices. It is possible to observe the migration in linear and stable concentration profiles for over 48 hours. As gradients are rapidly established, fast responses (occurring in less than 30 minutes) can also be measured.

The µ-Slide Chemotaxis 3D is for use with all adherent cells that will be observed in 2D or in in vivo-like 3D gel conditions, such as endothelial cells, fibroblasts, cancer cells, and others and non-adherent cells, such as T-cells, dendritic cells, neutrophils, monocytes, granulocytes, lymphocytes, and others.

Principle:

A narrow observation area connects two large reservoirs. The cells that are embedded in a gel inside the observation area become super-imposed by linear and time-stable gradients.

Chemotaxis is observed using time-lapse microscopy.

Observation area is recorded with a 4 x objective.

HT-1080 cells migrating towards FCS inside a gel matrix (objective lens 10 x).

Dendritic cell (mouse) with LifeAct-labeled F-actin that is migrating towards CCL 19 inside a Collagen I
gel matrix. Spinning disc confocal microscopy (objective lens 63 x).