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In our Application Note Chemotaxis (AN 14) you will find detailed information on how to perform your 2D chemotaxical assays using the µ-Slide Chemotaxis 2D.

ibidi FAQs

Find answers to frequently asked questions on chemotaxis assays.


Register for a 2 day Laboratory Course at ibidi Munich / Germany: Chemotaxis Assays and Video Microscopy.

µ-Slide Chemotaxis 2D

The μ-Slide Chemotaxis 2D is optimized to analyze the chemotactical response of adherent cells in linear and stable concentration profiles. Due to the gradient’s time stability of over 48 hours, it is the first system that is able to analyze the chemotaxis of slow migrating cells for up to two days.

The µ-Slide Chemotaxis 2D is for use with adherent cells only, such as: endothelial cells, fibroblasts, cancer cells, and others.


A narrow observation area connects two large reservoirs. The cells that are seeded into the observation area become super-imposed by linear and time-stable gradients.

Chemotaxis is observed using time-lapse microscopy. The ibidi Heating & Incubation Systems enable live cell imaging with incubator conditions directly on the microscope.

Observation area is recorded with a 4 x objective.

Chemotaxis of HT-1080 cells, which are migrating towards FCS, using low resolution microscopy for a maximum of migration data

High resolution microscopy during chemotaxis and cell polarization, using the HT-1080 LifeAct cell line