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ibidi FAQs

Find answers to frequently asked questions on chemotaxis assays.


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2D Versus 3D Chemotaxis

Example of HT-1080 cancer cells on a 2D surface (top), and embedded into a Collagen I gel (bottom)

It is commonly accepted that cells in a culture behave very differently when they are attached to a flat 2D surface, compared to being inside a 3D gel matrix. In most cases, the 3D environment mimics the in vivo situation much better. Gradients for chemotaxis can easily be built up in water-based gels (like Collagen I gels), because the gel structure does not hinder diffusion. The µ-Slide Chemotaxis is ideally suited for experiments in 3D environments.