ibidi MOVIE



Watch our movie Long-Term Cell Culture Under Perfusion (MV 21) to get more information on applications and handling.

ibidi TIP

Contact ibidi for a free demo of the ibidi Pump System.

 

ibidi WEBINAR

Listen to ibidi’s recorded webinar "Cell Cultivation under Flow".

PRACTICAL COURSE

Register for a 2 day Laboratory Course at ibidi Munich / Germany: Cell Cultivation under Perfusion and Live Cell Imaging.

ibidi FAQs

Find answers to frequently asked questions on cell culture under flow.

What Type of Assay Are You Planning?

Cell Culture under Shear Stress

Application:
Endothelial cells under flow

  • Investigating the influence of shear stress
    on endothelial cells
  • Preparing cells while mimicking in vivo perfusion conditions
  • Investigating platelet or lymphocyte adhesion to endothelial cells cultured under in vivo-like conditions
  • Creating biofilms of microorganisms
  • Staining antibodies

Flow Characteristics:

Continuous laminar, non-uniform, oscillating (needed for turbulence simulation), pulsatile

Duration:

Hours, up to several weeks

Experimental Environment:

Incubation conditions

Recommended Pumps:

ibidi Pump System

Recommended μ-Slides:

μ-Slide VI 0.4, μ-Slide I Luer Family, μ-Slide III 0.1

Rolling and Adhesion Assays

Application:
Rolling and adhesion of suspended cells to protein surfaces

  • Blood cells (e.g., platelets, leukocytes, and monocytes) on adhesion proteins
  • Blood cells on confluent cell monolayers

Flow Characteristics:

Continuous laminar

Duration:

Minutes to hours

Experimental Environment:

Room temperature or incubation conditions

Recommended Pumps:

ibidi Pump System, Syringe pumps, Peristaltic pump

Recommended μ-Slides:

μ-Slide VI 0.4, μ-Slide I Luer Family, μ-Slide y-shaped

Stop Flow Experiments

Application:
Defined liquid exchange

  • Ca2+-imaging
  • Defined medium exchange for optimal feeding
  • Online drug delivery
  • Live staining

Flow Characteristics:

Laminar flow with short periods of flow in between non-flow phases

Duration:

Minutes to hours

Experimental Environment:

Room temperature or incubation conditions

Recommended Pumps:

Manual liquid delivery, Syringe pumps, Peristaltic pump

Recommended μ-Slides:

μ-Slide VI 0.4, μ-Slide I Luer Family, μ-Slide I

3D Cell Culture: Interstitial Flow

Application:
3D cultures of cells and tissue in gel matrices:

  • Hepatocytes
  • Fibroblasts
  • Muscle cells
  • Kidney cells
  • Stem cells

Flow Characteristics:

Continuous interstitial or stop flow interstitial

Duration:

Hours, up to several weeks

Experimental Environment:

Incubation conditions

Recommended Pumps:

ibidi Pump System

Recommended μ-Slides:

μ-Slide VI 0.4, μ-Slide I Luer Family

ECIS Flow Assays 

ECIS technology permits the measurement of even small morphological changes in electrical signals, without disturbance to the cells, and without requiring any staining. The measurements are automated and are therefore highly reproducible with minimum effort.

The ECIS Flow Array enables researchers to investigate cells under both perfusion and defined shear stress conditions.