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APPLICATION NOTE
Find detailed information on how to prepare a Collagen I gel for 3D Cell Culture in Application Note 26.
3D Cell Microscopy
Numerous ibidi products are made for 3D applications. Examples of 3D matrices are collagen type I gels, fibrin gels, MatriGel TM*, or synthetic hydrogels. In 3D assays, cells are brought inside, or on top of, an in vivo-like gel matrix. As with all ibidi μ-Slides, cells are, in most cases, being analyzed using fluorescence microscopy.
| Cells in a 3D matrix with a chemical gradient for chemotaxis. Typical for lymphocytes, neutrophils, and T-cells. Use the μ-Slide Chemotaxis3D |
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| Cell spheroid (or micro tumor) on top of, or embedded in, a 3D matrix for long-term cultivation. The upper well provides nutrients by means of diffusion. Use the μ-Slide Angiogenesis |
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| Cells in 3D matrix with applied interstitial flow in a channel Use the μ-Slide VI 0.4 |
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*MatriGel is a registered trademark of Becton Dickinson.