FREE SAMPLES

Order a FREE SAMPLE to test ibidi’s µ-Slides, µ-Dishes, and µ-Plates with your experiments.

APPLICATION NOTE

In our Application Note Tube Formation (AN 19) you will find detailed information on how to setup a tube formation assay.

APPLICATION NOTE

ibidi’s Application Note Data Analysis of Tube Formation Assays (AN 27) shows you how to optimize the setup of angiogenesis assays, and how to analyze and interprete the data.

ibidi WEBINAR

Listen to ibidi’s recorded webinar "Tube Formation Assay for Studying the Effect of Drugs on Angiogenesis".

Data Acquisition and Analysis

Data Acquisition

Microscopy is used to evaluate the tube formation process. Depending on your focus of interest, this can either be done by using video microscopy (a continuous movie), or by observing images at distinct time points (e.g., after 6 hours and 12 hours). The ibidi Heating & Incubation Systems enable live cell imaging with incubator conditions directly on the microscope.


One well of the µ-Slide Angiogenesis, with HUVEC cells on Matrigel  after 12 hours of incubation.

Characteristic pattern of human endothelial cells (HUVEC), stained with Calcein.


Data Analysis

Tube Formation Image Analysis is a crucial step in angiogenesis assays. Wimasis, ibidi’s certified partner for quantitative image analysis, has developed the analysis solution WimTube to quantitatively evaluate the generation of cellular networks.

First, use the μ-Slide Angiogenesis to perform your tube formation experiments and acquire microscopy images. Then, simply upload the images to the image analysis platform and receive your results via E-mail.
 

Analysis data contains:

  • Total tube lengths, numbers, and statistics
  • Loop numbers, areas, and perimeters
  • Cell-covered area
  • Branching points