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Customer Feedback

Over more than a decade, ibidi has earned the trust of customers worldwide.

  

Dr. Christiane Wiesner

“We use LifeAct-TagRFP and LifeAct-TagGFP2 plasmids in primary human macrophages. The LifeAct probes put us in the position to detect F-actin-rich adhesion structures, without the drawback of disturbing their dynamics. The signal is bright and clear without the background of nonintegrated G-actin. LifeAct gives us the opportunity to highlight F-actin-enriched, cytoskeletal organizations, without the disadvantages of fluorophore-tagged actin overexpression.”

Dr. Christiane Wiesner
University Medical Center
Hamburg-Eppendorf
Hamburg
Germany

www.linderlab.de


Randy Van Der Ploeg, St. Boniface Research Centre, Winnipeg, Manitoba, Canada

“We are pleased with the ibiTreat 35 mm culture dishes. I have given some of your product to one of our grad students to try and he is amazed with the robust axonal outgrowth that he finds only after one day of seeding cells.  I have tested other dishes from your competitors and I did not get the reproducibility in growth characteristics like the ibiTreat µ-Dish.

The 35 mm dish holds up wonderfully to automated scanning over a wide scan area (essentially 98% of the growth surface area) which demonstrates the optical clarity and focal stability of the surface over such a wide area (1296 scan fields per well). I look forward to using more of the ibidi product lines in the future.”    

Randy Van Der Ploeg
Division of Neurodegenerative Disorders
St. Boniface Research Centre
Winnipeg, Manitoba
Canada

http://www.sbrc.ca/dnd/


Kaela Varberg, Indiana University, School of Medicine, Indianapolis, USA

“The µ-Slides Angiogenesis created by ibidi have significantly increased the efficiency and accuracy of the angiogenesis assays in our lab. Using the ibidi slides not only reduces the amount of time spent capturing images, but it also reduces the time it takes to quantify the data since the structures are in the same focal plane. Another added bonus is the reduced amount of matrigel required for each experiment which saves money!”    

Kaela Varberg
Indiana University
School of Medicine
Indianapolis
USA

http://www.iupui.edu/


Shane R. McSweeney, Cardiovascular Division, BHF Centre of Research Excellence, School of Medicine, King’s College London, United Kingdom

"The µ-Slide I Luer facilitates the culture of endothelial cells under defined complex flow patterns. We are particularly interested in the effects of oscillatory shear stress on redox signaling pathways, and the ibidi Air Pump and Fluidic Units are ideal systems for this purpose. The µ-Slides are convenient for both live and fixed cell imaging as well as the collection of protein and RNA samples and other cell analyses. The system has allowed us to easily investigate physiological shear patterns in vitro without a background in engineering.”

Shane R. McSweeney
Cardiovascular Division
BHF Centre of Research Excellence
School of Medicine
King’s College London
United Kingdom

http://www.kcl.ac.uk/medicine/research/divisions/cardio/bhf/index.aspx


Judith Cathcart, Advanced Optical Microscopy Facility, University Health Network, Toronto, Canada

"The ibidi chambers (Culture-Inserts) we used in our wound healing assays helped to keep the cell free area clean and consistent in size without damaging the ECM or leading edge of the cell fronts, making analysis of results more manageable and comparable. They're convenient and easy to use and their small size helps to conserve precious cells and culture materials. We'd certainly use them again.“

Judith Cathcart
Advanced Optical Microscopy Facility
University Health Network
Toronto
Canada

http://www.uhnresearch.ca/facilities/aomf.htm


Jason W. Bjork, PhD, 3M Corporate Research Materials Laboratory, St. Paul, USA

“The ibidi Culture-Inserts for wound healing assays provide a new line of testing for us. We wouldn't do traditional scratch assays at all because of the inherent variation, but with the new inserts we're able to provide many images to support our research. Our results are repeatable and reliable regardless of which researcher conducts the assay.”

Jason W. Bjork
3M Research Materials Laboratory
St. Paul
USA

www.3M.com


Ross Flockhart, PhD, Stanford School of Medicine, Stanford, USA

"The ibidi cell migration Culture-Inserts enabled me to perform highly reproducible and quantitative migration experiments. Since physical scratching isn't required, uniformity is maintained and adverse wounding responses are not an issue.

The online quantification tool WimScratch for the assay is also fantastic and it yields quantitative data from the images extremely quickly with little effort required. I'd definitely recommend it for migration studies.“

Ross Flockhart, PhD
Stanford School of Medicine
Department of Dermatology
Stanford
USA

http://khavarilab.stanford.edu/


PD Dr. Peter J. Hanley, University of Münster, Münster, Germany

"In the past 5 years, we have extensively used the ibidi µ-Slide Chemotaxis 2D to perform time-lapse images of primary mouse macrophages migrating in a chemoattractant gradient. We are most grateful that this device was developed, otherwise it would have been technically difficult to perform robust chemotaxis assays over long time periods (in our case, up to 14 h). By adding a little food coloring dye to the chemoattractant solution one can observe that the dye requires days to equilibrate with the opposing reservoir. Gradient kinetics can be measured by substituting the chemoattractant for a fluorescent dye (with similar molecular weight) and taking serial confocal fluorescence images in the observation area, the narrow channel which connects the two reservoirs.“

PD Dr. Peter Hanley
University of Münster
Institute of Molecular Cell Biology
Münster
Germany

http://www.uni-muenster.de/Biologie.AllgmZoo/


Dr. Juliane Merl, Helmholtz Zentrum München, Germany

„We used the Torpedo siRNA Transfection Reagent for the transfection of human retinal pigment epithelial cells and experienced a reproducible and efficient knock-down of our protein of interest; even with really low amounts of siRNA.“

Dr. Juliane Merl
Research Unit Protein Science
Helmholtz Zentrum München
Germany

www.helmholtz-muenchen.de/institute/abteilung-proteinanalytik-prot/index.html


Loïc Dupré, INSERM, Toulouse, France

"The ibidi µ-Slides Chemotaxis 2D and 3D have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.

I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"

Loïc Dupré PhD
INSERM UMR 1043
Purpan University Hospital
Toulouse
France

http://www.cptp.inserm.fr/


Sam Noppen, Rega Institute for Medical Research, Leuven, Belgium

"I used the ibidi µ-Plate Angiogenesis 96 well for bioluminescence measurements. The plate performed exceptionally well for this specific application, as it reduced my sample volume drastically and the optical quality was just superb."

Sam Noppen
Rega Institute for Medical Research, KU Leuven
Leuven
Belgium

http://www.kuleuven.be/rega/cmt/


Joanne Marrison, University of York, UK

"I plated and transfected cells on the ibidi µ-Slide 8 well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are 'slide' sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great."

Joanne Marrison
Imaging and Cytometry Laboratory (B/K051)
Bioscience Technology Facility, Department of Biology
University of York, York
United Kingdom

www.york.ac.uk/biology/tf


Matthew Taylor, PhD, Princeton University, Princeton, USA

"The 35 mm µ-Dish is invaluable for high resolution imaging performed in support of my work. The optical plastic is fantastic substrate for building compartmentalized neuronal cultures. Thank you for making great products.”

Matthew Taylor
Post-doctoral Fellow
Department of Molecular Biology
Princeton
University
Princeton
, NJ
USA
  

http://molbio.princeton.edu/


Dr. Esther G.L. Koh, National University of Singapore, Singapore

“ibidi made it much simpler for me to prepare cells for confocal microscopy and live-cell timelapse microscopy.  Cells that attached poorly to glass grew better on ibidi µ-Slides/Dishes.  The angiogenesis slide allowed me to minimise the amount of cells and reagents required for an experiment.”

Dr. Esther G.L. Koh
Head, Advanced Imaging Laboratory
Life
Sciences Institute Immunology Programme
National University of Singapore
Centre for Life Sciences
Singapore


Dr. Nynke van den Akker, Maastricht University, Maastricht, The Netherlands

“We've been working with the ibidi Pump System and Labware for over 5 years now and have recommended it to numerous colleagues as well. The plastics are ideal for cell culture and all forms of imaging, including automated imaging (easy to focus) and all sorts of fluorescence (clear, optically superb plastics). The ibidi Pump System in fact made the endothelial cell under flow the default of our lab! And above all, the technical support is always fast and efficient.”

Dr. Nynke van den Akker
Department of Cardiology
Maastricht
University
Maastricht
The Netherlands
www.maastrichtuniversity.nl/web/show/id=74913/langid=42


Owen McCarty, PhD, Oregon Health & Science University, Portland, USA

“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”

Owen McCarty, PhD
Associate Professor
Department of Biomedical Engineering
Oregon Health & Science University
Portland, Oregon
USA

http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/biomedical-engineering/


James J. Faust and David G. Capco, Arizona State University, Tempe, USA

“ibidi has made the tedious process of correlated light- and scanning electron microscopy  remarkably simplified with their µ-Dishes 35mm, high glass bottom Grid 500. This product combines all of the essential elements for high quality imaging: optically flawless glass essential for high resolution light microscopy, media reservoirs suited for a variety of imaging systems, and a grid system to rapidly relocate specimens in the scanning electron microscope. This product has made the difficult easy.”

James J. Faust and David G. Capco
Molecular and Cellular Biosciences
Arizona
State University
Tempe
, AZ
USA

http://mcb.asu.edu/


Dr. Thomas A.J. McKinnon, Imperial College London, UK

„I work with the ibidi slides because they are superior to any other product of this kind on the market. They are easy to use, give consistent results, economical and are suitable for a wide range of applications. The flow slides have without a doubt transformed my labs research and made many new experiments possible. Well done ibidi! I am indeed extremely happy with the ibidi products.”

Dr. Thomas A.J. McKinnon BSc, PhD
Post Doctoral Research Associate
Department of Hematology
Imperial College London
UK

http://www.imperial.nhs.uk/hammersmith/


Prof. Dr. Markus Sauer, University of Wuerzburg, Germany

„ibidi slides are compelling!
We tested the open μ-Slides with 8 wells for live-cell super-resolution imaging by dSTORM with SNAP-tags. The quality of the super-resolved images was impressive.“

Prof. Dr. Markus Sauer
Biotechnology & Biophysics
Julius-Maximilians-University Wuerzburg
Germany

http://www.super-resolution.biozentrum.uni-wuerzburg.de/research_topics/super_resolution_imaging/


Prof. Dr. Stefan Zahler, University of Munich, Germany

„Using the „angiogenesis slide“ from ibidi is the only possibility that I know for achieving a consistently good optical quality in the tube formation assay, and saving Matrigel at the same time.

In comparison to other systems, the heating stage from ibidi shows superior thermal stability and enables you to work with high humidity (>80%). This is not possible with other systems but is indispensable for long-term studies.“

Prof. Dr. Stefan Zahler
Pharmaceutical Biology
Munich Center for System-Based Drug Research
Ludwig-Maximilians-University
Germany

www.pharmbiol.cup.uni-muenchen.de


Jason Jacoby, University of Illinois, USA

„Actually, ibidi dishes have single-handedly revolutionized my science. The cells that I use (horizontal retinal cells from fish) are extremely intolerant to being plated on any form of glass, even if the glass is treated with several different compounds. Because of this issue, I was only able to perform fluorescent microscopy on upright confocal microscopes; they allowed us to plate our cells on plastic dishes and image from above. Now, with the ibidi dishes, we are able to use better quality inverted microscopes and higher powered objectives. Our results have proven strong, and we have mentioned the power of ibidi products in our most recent publication.

I am so happy that I learned of your great products through a colleague. Microscopy will never be the same for our laboratory. So far I have purchased 3 boxes of 35mm ibiTreat high dishes. Thank you very, very much.”

Jason Jacoby PhD Candidate
Laboratory of Neuroscience

University of Illinois at Chicago
USA

http://www.uic.edu/las/LIN


Prof. Dr. Klaus Palme, University of Freiburg, Germany

„The cooperation with ibidi made it possible to provide cell culture systems with a unique optical quality. The use of ibidi slides allowed for image acquisition in the necessary optical resolution, so that development processes in living proteoplasts of the model plant Arabidopsis thaliana could be explored. The µ-Slides allow monitoring of developmental processes in highest resolution. This enables us to use genetic techniques for recording and controlling the necessary processes of organ development.”

Prof. Dr. Klaus Palme
Institute for Biology II
Freiburg Institute for Advanced Studies FRIAS – School of Life Sciences
Albert-Ludwigs-University Freiburg
Germany

http://www.frias.uni-freiburg.de/lifenet