ContactUs@ibidiusa.com 1-800-372-6102
  • Home
  • Products
    • ibidi Labware
      • Open Slides | Dishes: ibidi Standard Bottom
        • µ-Dish 35mm, high
        • µ-Dish 35mm, low
        • µ-Dish 50mm, low
        • µ-Dish 35mm Grid-500
        • µ-Slide 2 well
        • µ-Slide 2 well Ph+
        • µ-Slide 4 well
        • µ-Slide 4 well Ph+
        • µ-Slide 8 well
        • µ-Slide Angiogenesis
        • µ-Slide 2 x 9 well
        • µ-Slide 18 well - flat
        • DIC Lid
      • Open Slides | Dishes: Glass Bottom
        • µ-Dish 35 mm, high glass bottom
        • µ-Dish 35 mm, high glass bottom Grid-50
        • µ-Dish 35 mm, high glass bottom Grid-500
        • µ-Dish 35 mm, high ESS
      • Open Slides | Dishes: Removable Chambers
        • Culture-Insert Family
        • micro-Insert 4 well Family
        • µ-Chamber 12 well
      • Channel Slides
        • µ-Slide I
        • µ-Slide I Luer Family
        • µ-Slide VI 0.1
        • µ-Slide VI 0.4
        • µ-Slide Chemotaxis 2D
        • µ-Slide Chemotaxis 3D
        • µ-Slide y-shaped
        • µ-Slide III 0.1
        • µ-Slide III 3in1
        • µ-Slide VI - flat
      • Flow Accessories
        • µ-Slide I Luer Flow Kit
        • µ-Slide y-shaped Flow Kit
        • µ-Slide VI 0.1 Flow Kit
        • µ-Slide VI 0.4 Flow Kit
        • µ-Slide III 0.1 Flow Kit
        • µ-Slide III 3in1 Flow Kit
        • µ-Slide upright 0.8 Flow Kit
        • Elbow Luer Connector
        • Luer Connector Male
        • Luer Lock Connector Male
        • Luer Lock Connector Female
        • Luer Plug Male
        • In-line Luer Injection Port
        • Serial Connector
        • Silicone Tubing
        • Female Luer Lock Coupler
        • Y Tube Fitting 0.8 mm
        • Y Tube Fitting 1.6 mm
        • Hose Clip
        • Fitting Reducer 0.5 to 1.6
      • sticky-Slides
        • sticky-Slide 8 well
        • sticky-Slide I Luer
        • sticky-Slide VI 0.4
        • sticky-Slide Chemotaxis 3D
      • µ-Plates
        • µ-Plate 24 well
        • µ-Plate 96 well
        • µ-Plate 384 well
        • µ-Plate Angiogenesis 96 well
      • Customer Specific Slides
        • Custom Specific Flow Slides and Channels
        • Custom Specific µ-Slide I Luer Electrode
    • Instruments & Accessories
      • Heating & Incubation Systems
        • ibidi Heating System 1, Universal Fit
        • ibidi Heating System 2, for Multi-Well Plates
        • ibidi Heating Stages Round Format
        • ibidi Gas Incubation System
      • Pump Systems
        • ibidi Pump System
        • Syringe Pumps
      • Cell Culture Incubator
      • ECIS (Electric Cell-Substrate Impedance Sensing)
        • ECIS System
        • ECIS Cultureware
        • ECIS Instrument Selection Guide
      • Accessories
        • µ-Dish Microscopy Rack
        • µ-Slide Microscopy Rack
        • µ-Slide Rack
        • µ-Slide Click Rack
        • Olaf Humidifiying Chamber
    • Cells & Reagents
      • LifeAct TM Actin Visualization
        • LifeAct Plasmids
        • LifeAct Adenoviral Vectors
        • HT-1080 LifeAct-TagGFP2 Stable Cell Line
        • Cell Line Services
      • Transfection Reagents
        • Torpedo DNA Transfection Reagent
        • µ-Transfection Kit VI
        • µ-Transfection Kit VI FluoR
        • µ-Proteofection Kit
      • Adenovirus Transduction
        • ibiClone TM Adeno Adenovirus Cloning Kit
        • ibiPure TM Adeno Adenovirus Purification Kit
        • ibiBoost TM Adeno Adenovirus Transduction Enhancer
      • ibidi Freezing Medium
      • ibidi Mounting Medium
      • ibidi Anti-Evaporation Oil
      • Collagen Type I
    • Software and Image Analysis
      • Automated Image Analysis
        • Wound Healing Image Analysis - WimScratch
        • Tube Formation Image Analysis - WimTube
        • Chemotaxis Image Analysis - WimTaxis
        • Sprouting Assay Image Analysis - WimSprout
        • Cell Counting Image Analysis - WimCounting
        • Chorioallantoic Membrane Assay Image Analysis - WimCAM
        • Cytotoxicity Image Analysis - WimCytotoxicity
        • Comet Assay Image Analysis - WimComet
        • Colony-Forming Image Analysis - WimColony
        • Neurite Outgrowth Image Analysis – WimNeuron
        • Retina Vessel Image Analysis – WimRetina
        • Transfection Efficiency Image Analysis – WimTransfection
      • Manual Image Analysis
        • Chemotaxis and Migration Tool
        • Manual Tracking
      • Software Solutions
        • PumpControl Software
    • Products by Applications
      • Live Cell Imaging
        • In-vivo Conditions on the Microscope
        • Fluorescent Markers for Actin Visualization
        • Transfection, Transduction and Proteofection
      • Cell-Based Microscopy Assays
        • Cell-Based Microscopy Assays
        • Immuno­fluorescence
        • Screening
      • Functional Cell-Based Assays
        • Wound Healing and Migration
        • Angiogenesis
        • Chemotaxis
        • Cell Culture Under Shear Stress
      • Impedance-Based Cell Assays
      • Image Analysis
        • Automated Image Analysis
        • Manual Image Analysis
  • Applications
    • Technical Aspects of Microscopy
      • Optical Properties
      • Cell Culture Surfaces
      • Compatibility with Microscopy Techniques
      • Cell Culture Geometry
    • Live Cell Imaging
      • Cell Culture Environment on the Microscope
      • In vivo-like Cell Culture Conditions
      • Optimized Optical Conditions
      • Experimental Examples
    • Fluorescent Markers for Actin Visualization
      • F-Actin’s Role in Cellular Processes
      • Actin Staining Techniques
      • Advantages of Using LifeAct
    • Transfection, Transduction, and Proteofection
      • Transfection
      • Transduction
      • Proteofection
    • Cell-Based Microscopy Assays
      • 3D Cell Microscopy
      • Co-Cultivation
      • Small Organisms
      • Stem Cell Differentiation
      • Specific Substrates
      • Micromanipulation
    • Immunofluorescence (IF)
      • Can Immunofluorescence be Fun?
      • Eliminate the Need for Tweezers in IF Protocols
      • Technical Details Comparison of Various IF Applications
      • All-in-One Channel Formats for IF
      • All-in-One Open Well Formats for IF
      • Removable Chambers for IF
      • Application Examples
    • Screening
      • Tube Formation Assays in the µ-Plate Angiogenesis 96 well
      • Impedance Based Wound Healing Assays using ECIS
      • Screening in ibidi’s Multi-well Plates
    • Wound Healing and Migration
      • Experimental Workflow
      • Handling of Culture-Inserts
      • Data Acquisition and Analysis
      • Types of Migration Assays
      • Comparison to Scratch Assay
      • Impedance Measurements
    • Angiogenesis
      • Experimental Workflow
      • Easy Handling
      • Data Acquisition and Analysis
      • Types of Angiogenesis Assays
    • Chemotaxis
      • Experimental Workflow
      • µ-Slide Chemotaxis 2D
      • µ-Slide Chemotaxis 3D
      • Gradient Stability
      • 2D Versus 3D Chemotaxis
      • Data Analysis
      • Chemotaxis and Migration Tool
      • Chemotaxis Parameters
      • Types of Chemotaxis Assays
      • Example Experiment
    • Cell Culture under Shear Stress
      • What Type of Shear Stress Do You Investigate?
      • How to Identify the Ideal Pump for Your Experiment
      • What Type of Assay Are You Planning?
      • Setting Up a Flow Experiment
    • Impedance-Based Cell Assays
      • Experimental Workflow
      • Working Principle
      • Wound Healing and Cell Migration
      • Cell Attachment
      • Measurement and Modeling of Endothelial Cell Barrier Function
      • Flow Assays
      • Toxicological Screening
      • Measurement of Metastatic Potential
      • Signal Transduction
  • Support
    • Instructions / Manuals
      • µ-Slides / µ-Dishes / µ-Plates
      • Instruments & Accessories
      • Cells & Reagents
      • Software & Image Analysis
    • Application Notes
      • Use of µ-Slides
      • Cell-Based Assays
      • Cell Culture Applications
      • Application Notes in Numerical Order
    • Movies
      • Use of µ-Slides
      • Cell-Based Assays
      • Cell Culture Applications
      • Movies in Numerical Order
    • Material Safety Data Sheets
    • References
    • Literature
    • Frequently Asked Questions (FAQ)
      • Light Microscopy
      • Live Cell Imaging
      • µ-Slides and µ-Dishes
      • LifeAct Actin Visualization
      • Adenovirus Transduction
      • Chemotaxis Assays
      • Wound Healing and Migration Assays
      • Tube Formation Assays
      • Cell Culture under Flow
      • Image Analysis
    • Technical Support
    • Webinars
      • Recorded Webinars
    • Practical Courses
  • Order / Contact
    • Online Ordering
    • Headquarter Germany
    • Europe
    • North America
    • South America
    • Asia
    • Australia
    • Africa
    • Distributor Area
    • Free Sample Program
  • Events
    • Congresses & Trade Shows
      • North America
      • Europe
      • Asia-Pacific
      • Archive
    • Practical Courses
    • Webinars
      • Recorded Webinars
  • About ibidi
    • Profile
      • Company Overview
      • History
      • The ibidi Brand
      • Awards & Partnerships
    • Customer Feedback
    • Career
    • Press Releases
      • Press Releases English
      • Press Releases German
    • How to Reach Us
      • Using Public Transportation
      • Traveling by Taxi
      • Arriving by Car
    • Congresses & Trade Shows
      • North America
      • Europe
      • Asia-Pacific
      • Archive
Loading
  • Applications
  • Technical Aspects of Microscopy

Open Slides / Dishes Standard Bottom

Open Slides / Dishes Glass Bottom

Channel Slides

µ-Plates

Applications
  • Technical Aspects of Microscopy
    • Optical Properties
    • Cell Culture Surfaces
    • Compatibility with Microscopy Techniques
    • Cell Culture Geometry
  • Live Cell Imaging
  • Fluorescent Markers for Actin Visualization
  • Transfection, Transduction, and Proteofection
  • Cell-Based Microscopy Assays
  • Immunofluorescence (IF)
  • Screening
  • Wound Healing and Migration
  • Angiogenesis
  • Chemotaxis
  • Cell Culture under Shear Stress
  • Impedance-Based Cell Assays

QUICK LINKS

  • What's New
  • Special Offers
  • All Products
  • eCatalog
  • Webinars
  • Practical Courses
  • Application Notes
  • ibidi Movies

Technical Aspects of Microscopy

What are the important optical properties of high resolution microscopy?

  • No. 1.5 Coverslip-Like Thickness
  • Compatibility to Immersion Oil
  • Standard Refractive Index
  • Numerical Aperture
  • Material Dispersion /
    Abbe Number > 55
  • Transmission
  • Low Autofluorescence

 

Which cell culture surface is best suited for me?

  • ibiTreat – The ibidi Standard Surface
  • Other Surfaces and Coatings
  • ibidi Special Surfaces
  • Compatibility of Surfaces with Microscopy Techniques

 

How does cell culture geometry influence HR microscopy?

  • Chamber Geometry
  • All-in-One Chambers
  • Compatibility of ibidi Products with Upright or Inverted Microscopes
  • Homogeneous Cell Distribution, as a Result of Geometry
  • Phase Contrast in Channel Slides is Superior to Small Open Wells

 

Are you looking for protocols, application notes, or practical movies?

  • Application Note 08: Cell Culture Coating
  • Application Note 03: Growing Cells in µ-Channels
  • Movie 01: Seeding Cells in µ-Channels
  • Sitemap
  • Privacy Policy
  • AGB
  • Legal Notes
  • Impressum
Copyright © 2013 ibidi GmbH. All rights reserved. Certified ISO 9001, ISO 13485